Anticorps Polyclonal de lapin anti-Aurora A

• Phare
• Validé par KD/KO

Aurora A Polyclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 10297-1-AP

Synonymes

AIK, ARK 1, ARK1, AURA, AURKA, Aurora A, aurora kinase A, Aurora related kinase 1, Aurora/IPL1 related kinase 1, AURORA2, Breast tumor amplified kinase, BTAK, hARK1, STK15, STK6, STK7

---

Galerie de données de validation

• 

  IHC staining of human breast cancer using 10297-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10297-1-AP (Aurora A antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10297-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10297-1-AP (Aurora A antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10297-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10297-1-AP (Aurora A antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10297-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10297-1-AP (Aurora A antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10297-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10297-1-AP (Aurora A antibody) at dilution of 1:10 (under 25x lens).

• 

  IF Staining of HeLa using 10297-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 10297-1-AP (Aurora A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10297-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 10297-1-AP (Aurora A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10297-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 10297-1-AP (Aurora A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Informations sur le produit

10297-1-AP cible Aurora A dans les applications de IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Aurora A Protéine recombinante Ag0362
• Nom complet: aurora kinase A
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC002499
• Symbole du gène: AURKA
• Identification du gène (NCBI): 6790
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).

Publications

Species	Application	Title
human		Acta Pharm Sin B; Sinomenine ester derivative inhibits glioblastoma by inducing mitochondria-dependent apoptosis and autophagy by PI3K/AKT/mTOR and AMPK/mTOR pathway.; Authors - Xiangjin Zheng
mouse	IF	Nat Commun; CPEB1 directs muscle stem cell activation by reprogramming the translational landscape.; Authors - Wenshu Zeng
human		Oncotarget; Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas.; Authors - Yanwei Liu
human		Eur J Med Chem; Design, synthesis, biological evaluation of 6-(2-amino-1H-benzo[d]imidazole-6-yl)quinazolin-4(3H)-one derivatives as novel anticancer agents with Aurora kinase inhibition.; Authors - Chengcheng Fan
human		Front Immunol; The ALDH Family Contributes to Immunocyte Infiltration, Proliferation and Epithelial-Mesenchymal Transformation in Glioma.; Authors - Zeyu Wang
human		J Exp Clin Cancer Res; 3-O-acetyl-11-keto-β-boswellic acid exerts anti-tumor effects in glioblastoma by arresting cell cycle at G2/M phase.; Authors - Wan Li