Anticorps Monoclonal anti-AURKA

• Phare
• Validé par KD/KO

AURKA Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG3

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(21)

CloneNo.

1F4B10

Avis (1)

N° de cat : 66757-1-Ig

Synonymes

AIK, ARK 1, ARK1, AURA, AURKA, aurora kinase A, Aurora related kinase 1, Aurora/IPL1 related kinase 1, AURORA2, Breast tumor amplified kinase, BTAK, hARK1, STK15, STK6, STK7

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Galerie de données de validation

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  WB analysis of human testis using 66757-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66757-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 66757-1-Ig

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 66757-1-Ig

  rat testis tissue were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66757-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 66757-1-Ig

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66757-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66757-1-Ig (AURKA antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66757-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66757-1-Ig (AURKA antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66757-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using AURKA antibody (66757-1-Ig, Clone: 1F4B10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain, cellules HeLa, cellules NCCIT, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 18 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

66757-1-Ig cible AURKA dans les applications de WB, IHC, IF/ICC, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG3
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AURKA Protéine recombinante Ag20096
• Nom complet: aurora kinase A
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 46-52 kDa
• Numéro d’acquisition GenBank: BC002499
• Symbole du gène: AURKA
• Identification du gène (NCBI): 6790
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).

Publications

Species	Application	Title
human	WB	Front Oncol; PUF60/AURKA Axis Contributes to Tumor Progression and Malignant Phenotypes in Bladder Cancer.; Authors - Qian Long
human	IHC	Front Oncol; Integrated Analysis of Transcriptome Data Revealed AURKA and KIF20A as Critical Genes in Medulloblastoma Progression.; Authors - Bo Liang
human	WB	Front Oncol; Proteomics and phosphoproteomics of chordoma biopsies reveal alterations in multiple pathways and aberrant kinases activities; Authors - Jing Hang ; KD Validated
human	WB	Biochem Pharmacol; Palmatine induces G2/M phase arrest and mitochondrial-associated pathway apoptosis in colon cancer cells by targeting AURKA.; Authors - Xiaojiang Liu
human	WB	Carcinogenesis; ARID3A promotes the development of colorectal cancer by upregulating AURKA.; Authors - Jing Tang
human	WB	Biochim Biophys Acta Mol Basis Dis; Blocking AURKA with MK-5108 attenuates renal fibrosis in chronic kidney disease.; Authors - Mingzhu Jiang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sarah-Eve (Verified Customer) (06-02-2023)

Works well, WB dilution 1:2000

• Applications: Western Blot
• Cell Tissue Type: Human pulmonary artery smooth muscle cells

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