Anticorps Monoclonal anti-AURKA

• Phare
• Validé par KD/KO

AURKA Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG3

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F4B10

N° de cat : 66757-1-PBS

Synonymes

Aurora A, 1F4B10, AIRK1, ARK-1, AURA

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Galerie de données de validation

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  WB analysis of human testis using 66757-1-Ig (same clone as 66757-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

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  WB analysis using 66757-1-Ig (same clone as 66757-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat testis using 66757-1-Ig (same clone as 66757-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 66757-1-Ig (same clone as 66757-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66757-1-Ig (same clone as 66757-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

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  WB analysis of NCCIT using 66757-1-Ig (same clone as 66757-1-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66757-1-Ig (same clone as 66757-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66757-1-Ig (AURKA antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66757-1-Ig (same clone as 66757-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66757-1-Ig (AURKA antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66757-1-Ig (same clone as 66757-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using AURKA antibody (66757-1-Ig, Clone: 1F4B10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50265-1

  Cytometric bead array standard curve of MP50265-1, AURKA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66757-2-PBS. Detection antibody: 66757-1-PBS. Standard:Ag20096. Range: 0.391-50 ng/mL.

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Informations sur le produit

66757-1-PBS cible AURKA dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG3
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AURKA Protéine recombinante Ag20096
• Nom complet: aurora kinase A
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 46-52 kDa
• Numéro d’acquisition GenBank: BC002499
• Symbole du gène: AURKA
• Identification du gène (NCBI): 6790
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).

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