Anticorps Monoclonal anti-Aurora B

Aurora B Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(4)

CloneNo.

3H1

N° de cat : 60098-1-Ig

Synonymes

AIK2, AIM 1, AIM1, ARK 2, ARK2, AurB, AURKB, Aurora B, aurora kinase B, Aurora related kinase 2, Aurora/IPL1 related kinase 2, IPL1, STK 1, STK12, STK5

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Galerie de données de validation

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  WB analysis of HeLa using 60098-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60098-1-Ig (Aurora B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 60098-1-Ig

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 60098-1-Ig (Aurora B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 60098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60098-1-Ig (Aurora B antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 60098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60098-1-Ig (Aurora B antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu de thymus de souris
• Résultats positifs en IHC: tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

60098-1-Ig cible Aurora B dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Aurora B Protéine recombinante Ag7532
• Nom complet: aurora kinase B
• Masse moléculaire calculée: 39.2 kDa
• Poids moléculaire observé: 39-45 kDa
• Numéro d’acquisition GenBank: BC013300
• Symbole du gène: AURKB
• Identification du gène (NCBI): 9212
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Aurora B(AURKB) is also named as aurora kinase B, AIK2, AIM1, AIRK2, ARK2, STK1, STK12, STK5 and belongs to the Aurora subfamily. Aurora kinase B is a chromosomal passenger serine/threonine protein kinase that regulates accurate chromosomal segregation, cytokinesis, protein localization to the centromere and kinetochore, correct microtubule-kinetochore attachments, and regulation of the mitotic checkpoint(PMID:18311747). Expression levels of AURKB are increased in the anaplastic thyroid carcinoma cell lines ARO, Cal 62, and KAT-4(PMID:15562011).

Publications

Species	Application	Title
human	WB	Gastroenterology; Overexpression of Rac GTPase Activating Protein 1 Contributes to Proliferation of Cancer Cells by Reducing Hippo Signaling to Promote Cytokinesis.; Authors - Xiao-Mei Yang
mouse	WB	Hum Mol Genet; Downregulation of Survivin contributes to cell-cycle arrest during postnatal cardiac development in a severe spinal muscular atrophy mouse model.; Authors - Lei Sheng
human	WB	PLoS One; Anti-pancreatic cancer deliverables from sea: first-hand evidence on the efficacy, molecular targets and mode of action for multifarious polyphenols from five different brown-algae.; Authors - Aravindan Sheeja S
human	WB	FEBS J; YAP co-localizes with the mitotic spindle and midbody to safeguard mitotic division in lung-cancer cells; Authors - Shu-Er Chow

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