Anticorps Polyclonal de lapin anti-AXIN1

• Phare
• Validé par KD/KO

AXIN1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 16541-1-AP

Synonymes

AXIN, axin 1, AXIN1, Axis inhibition protein 1, hAxin

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Galerie de données de validation

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  WB analysis of HT-1080 using 16541-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 16541-1-AP (AXIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 16541-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16541-1-AP (AXIN1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 16541-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16541-1-AP (AXIN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 16541-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16541-1-AP (AXIN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 16541-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16541-1-AP (AXIN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HT-1080, cellules HEK-293T, cellules HeLa
• Résultats positifs en IHC: tissu de côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 11 publications below

Informations sur le produit

16541-1-AP cible AXIN1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AXIN1 Protéine recombinante Ag9858
• Nom complet: axin 1
• Masse moléculaire calculée: 826aa,92 kDa; 862aa,95 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC044648
• Symbole du gène: AXIN1
• Identification du gène (NCBI): 8312
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Axis inhibition protein1 (AXIN1), also called AXIN, together with AXIN2 are multidomain scaffold proteins that negatively regulate Wnt signaling. AXIN1 is likely to function as a tumor suppressor. Under UV irradiation, AXIN1-HIPK2-TP53 complex forms. The complex also controls cell growth, apoptosis and development. Like AXIN2, AXIN1 undergoes poly(ADP-ribosy)lation by tankyrase TNKS and TNKS2 followed by unbiquitination by RNF146 which leads to its degradation and subsequent activation of Wnt signaling. Its deubiquitination by USP34 is important for nuclear accumulation during Wnt signaling. Recent researches find that CircAXIN1 encodes a novel protein, AXIN1-295aa, which shows at around 40-55 kDa by Western Blot. AXIN1-295aa functions as an oncogenic protein, activating the Wnt signaling pathway to promote GC tumorigenesis and progression, suggesting a potential therapeutic target for GC. Proteintech's AXIN1 antibody 16541-1-AP is a rabbit polyclonal antibdy raised against the N-terminus of human AXIN1.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; AXIN2 Reduces the Survival of Porcine Induced Pluripotent Stem Cells (piPSCs).; Authors - Rui Zhang
human	WB	Front Oncol; Targeting TRIM54/Axin1/β-Catenin Axis Prohibits Proliferation and Metastasis in Hepatocellular Carcinoma.; Authors - Jinrong Zhu
human	WB	Cancer Gene Ther; Stabilizing and upregulating Axin with tankyrase inhibitor reverses 5-fluorouracil chemoresistance and proliferation by targeting the WNT/caveolin-1 axis in colorectal cancer cells.; Authors - Feng Luo
human	WB	Commun Biol; Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner.; Authors - Xihua Yue
human	WB	Exp Cell Res; The transcript ENST00000444125 of lncRNA LINC01503 promotes cancer stem cell properties of glioblastoma cells via reducing FBXW1 mediated GLI2 degradation.; Authors - Pan Wei
		FEBS Lett; Exosomes derived from mineralizing osteoblasts promote ST2 cell osteogenic differentiation by alteration of microRNA expression.; Authors - Yazhou Cui