Anticorps Polyclonal de lapin anti-AXL

AXL Polyclonal Antibody for FC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

22

Avis (1)

N° de cat : 13196-1-AP

Synonymes

AXL, AXL oncogene, AXL receptor tyrosine kinase, JTK11, UFO

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Galerie de données de validation

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  WB analysis using 13196-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13196-1-AP (AXL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13196-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13196-1-AP (AXL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13196-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13196-1-AP (AXL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13196-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13196-1-AP (AXL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 13196-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 13196-1-AP (AXL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13196-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13196-1-AP (AXL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 13196-1-AP

  IP result of anti-AXL(IP:13196-1-AP, 4ug; Detection:13196-1-AP 1:3000) with HeLa cells lysate 1320 ug.

• 

  FC experiment of HeLa using 13196-1-AP

  1X10^6 HeLa cells were stained with 0.2ug AXL antibody (13196-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules DU 145, cellules HeLa, cellules HepG2, cellules HT-1080, tissu hépatique humain
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 19 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• FC: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

13196-1-AP cible AXL dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AXL Protéine recombinante Ag3898
• Nom complet: AXL receptor tyrosine kinase
• Masse moléculaire calculée: 894 aa, 98 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC032229
• Symbole du gène: AXL
• Identification du gène (NCBI): 558
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Axl, also known as Ufo or Ark, is a receptor tyrosine kinase of the TAM (Tyro3, Axl and Mer) family. Axl is a single-pass type I membrane protein containing two fibronectin type-III domains, two Ig-like C2-type domains and one protein kinase domain. Axl mRNA is widely expressed with highest levels in the heart and skeletal muscle. Gas6 has been identified as a ligand for human Axl. Gas6/Axl signaling plays a role in cell survival, cell proliferation, migration and differentiation. It has been reported that 110-120 kDa non-glycosylated AXL protein can be detected(PMID: 31171001). Endogenous Axl is auto-phosphorylated and reacted with a tyrosine-phosphorylated protein of 140 kDa(PMID: 12730092).

Publications

Species	Application	Title
human	WB	Int J Cancer; Therapeutic Activity of DCC-2036, a Novel Tyrosine Kinase Inhibitor, against Triple-Negative Breast Cancer Patient-Derived Xenografts by Targeting AXL/MET.; Authors - Yingying Shen
rat	WB	Int J Mol Sci; Embryonic-Derived Myb- Macrophages Enhance Bacterial Clearance and Improve Survival in Rat Sepsis.; Authors - Mirjana Jerkic
human	WB	Int J Mol Sci; Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.; Authors - Elisa De Tomi
	FC	Bioorg Chem; Non-volatile acylphloroglucinol components from Eucalyptus robusta inhibit Zika virus by impairing RdRp activity of NS5.; Authors - Zhai-Wen Yao
human	WB, IHC,IF	Cancer Gene Ther; Targeting LRIG2 overcomes resistance to EGFR inhibitor in glioblastoma by modulating GAS6/AXL/SRC signaling.; Authors - Minhai Dong
human	WB	iScience; SUFU suppresses ferroptosis sensitivity in breast cancer cells via Hippo/YAP pathway.; Authors - Kun Fang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Marta (Verified Customer) (05-30-2022)

The antibody works well. Non-specific bands are not detected.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: A375 melanoma cell line

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