Anticorps Recombinant de lapin anti-AXL

• Phare
• Validé par KD/KO

AXL Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242014B9

N° de cat : 84659-1-RR

Synonymes

242014B9, EC:2.7.10.1, Tyrosine-protein kinase receptor UFO, UFO

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Galerie de données de validation

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  WB analysis using 84659-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84659-1-RR (Axl antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 84659-1-RR

  WB result of AXL antibody (84659-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AXL transfected HeLa cells.

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  IF Staining of HeLa using 84659-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using AXL antibody (84659-1-RR, Clone: 242014B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules A549, cellules NCI-H1299, cellules U2OS
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84659-1-RR cible AXL dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: AXL Protéine recombinante Eg1618
• Nom complet: AXL receptor tyrosine kinase
• Masse moléculaire calculée: 98kDa
• Poids moléculaire observé: 130-140 kDa
• Numéro d’acquisition GenBank: NM_021913.4
• Symbole du gène: AXL
• Identification du gène (NCBI): 558
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Axl, also known as Ufo or Ark, is a receptor tyrosine kinase of the TAM (Tyro3, Axl and Mer) family. Axl is a single-pass type I membrane protein containing two fibronectin type-III domains, two Ig-like C2-type domains and one protein kinase domain. Axl mRNA is widely expressed with highest levels in the heart and skeletal muscle. Gas6 has been identified as a ligand for human Axl. Gas6/Axl signaling plays a role in cell survival, cell proliferation, migration and differentiation. It has been reported that 110-120 kDa non-glycosylated AXL protein can be detected(PMID: 31171001). Endogenous Axl is auto-phosphorylated and reacted with a tyrosine-phosphorylated protein of 140 kDa(PMID: 12730092).

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