Anticorps Monoclonal anti-AZI2

AZI2 Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

2F1G5

Avis (1)

N° de cat : 68326-1-Ig

Synonymes

5 azacytidine induced 2, AZ2, AZI2, Nak associated protein 1, NAP1, TILP

---

Galerie de données de validation

• 

  WB analysis using 68326-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68326-1-Ig (AZI2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  IF Staining of HeLa using 68326-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using AZI2 antibody (68326-1-Ig, Clone: 2F1G5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules U2OS, cellules 4T1, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules K-562, cellules LNCaP, cellules MDA-MB-231
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68326-1-Ig cible AZI2 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AZI2 Protéine recombinante Ag6755
• Nom complet: 5-azacytidine induced 2
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC001139
• Symbole du gène: AZI2
• Identification du gène (NCBI): 64343
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AZI2, also known as NAP1, is a TNF receptor (TNFR)-associated factor family member-associated NF-κB activator-binding kinase 1-binding protein that regulates the production of IFNs. It is an adapter protein which binds TBK1 and IKBKE playing a role in antiviral innate immunity. NAP1 is a kind of adapter protein which binds TBK1 and IKBKE playing a role in antiviral innate immunity. (PMID: 21931631)

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Muhammed Talha (Verified Customer) (08-31-2023)

I tested this antibody in WT vs NAP1 KO cells. It gave me 2 bands. One of them is actual band and other one is nonspecific one I guess. They are very close to each other and hard to separate them unfortunately.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: hela

AZI2 Antibody Western Blot validation (1:10000 dilution) in hela (Cat no:68326-1-Ig)