Anticorps Recombinant de lapin anti-Acetyl-Histone H2B (Lys12)

Acetyl-Histone H2B (Lys12) Recombinant Antibody for WB, Dot blot, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, Dot blot, ELISA

Conjugaison

Non conjugué

CloneNo.

250005E8

N° de cat : 86731-1-RR

Synonymes

HIST2H2BE, Histone H2B, GL105, H2B, H2B.1

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 86731-1-RR

  Trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 86731-1-RR (Acetyl-Histone H2B (Lys12) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody (60004-1-Ig) as loading control.

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  WB analysis of HeLa using 86731-1-RR

  Trichostatin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 86731-1-RR (Acetyl-Histone H2B (Lys12) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody (60004-1-Ig) as loading control.

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  Dot Blot experiment of / using 86731-1-RR

  Dot blot analysis was used to confirm the specificity of 86731-1-RR Acetyl-Histone H2B (Lys12) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

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Applications testées

• Résultats positifs en WB: Trichostatin A treated NIH/3T3 cells, Trichostatin A treated HeLa cells
• Résultats positifs en Dot blot: / /,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• DOT BLOT: DOT BLOT : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86731-1-RR cible Acetyl-Histone H2B (Lys12) dans les applications de WB, Dot blot, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: histone cluster 2, H2be
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC005827
• Symbole du gène: Histone H2B
• Identification du gène (NCBI): 8349
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

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