Anticorps Recombinant de lapin anti-Acetyl-Histone H2B (Lys15)

Acetyl-Histone H2B (Lys15) Recombinant Antibody for WB, Dot Blot, ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, Dot Blot, ELISA, ChIP-qPCR

Conjugaison

Non conjugué

CloneNo.

250366G3

N° de cat : 83171-7-RR

Synonymes

Ac Histone H2B, Ac Histone H2B (Lys15), Ac Histone H2B Lys15, Ac Histone H2B-Lys15, Acetyl Histone

---

Galerie de données de validation

• 

  WB analysis of NIH/3T3 using 83171-7-RR

  Trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83171-7-RR (Acetyl-Histone H2B (Lys15) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Beta Actin Monoclonal antibody (66009-1-Ig) as loading control.

• 

  Dot Blot experiment using 83171-7-RR

  Dot blot analysis was used to confirm the specificity of 83171-7-RR Acetyl-Histone H2B (Lys15) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

• 

  ChIP experiment of HeLa using 83171-7-RR

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H2B (Lys15) (83171-7-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

---

Applications testées

• Résultats positifs en WB: Trichostatin A treated NIH/3T3 cells,
• Résultats positifs en Dot Blot: N/A,
• Résultats positifs en ChIP-qPCR: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• DOT BLOT: DOT BLOT : 1:500-1:2000
• CHImmunoprécipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83171-7-RR cible Acetyl-Histone H2B (Lys15) dans les applications de WB, Dot Blot, ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: histone cluster 2, H2be
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC005827
• Symbole du gène: Histone H2B
• Identification du gène (NCBI): 8349
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

{{ptg:RelatedPrimaryAntibodies}}