Anticorps Recombinant de lapin anti-Acetyl-Histone H2B (Lys20)

Acetyl-Histone H2B (Lys20) Recombinant Antibody for WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR

Conjugaison

Non conjugué

CloneNo.

241204H8

N° de cat : 84549-1-RR

Synonymes

Ac Histone H2B, Ac Histone H2B (Lys20), Ac Histone H2B Lys20, Ac Histone H2B-Lys20, Acetyl Histone

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 84549-1-RR

  Trichostatin A treated and untreated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84549-1-RR (Acetyl-Histone H2B (Lys20) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

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  IF Staining of HeLa using 84549-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H2B (Lys20) antibody (84549-1-RR, Clone: 241204H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of NIH/3T3 using 84549-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated NIH/3T3 cells using Acetyl-Histone H2B (Lys20) antibody (84549-1-RR, Clone: 241204H8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HeLa using 84549-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated HeLa cells using Acetyl-Histone H2B (Lys20) antibody (84549-1-RR, Clone: 241204H8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Dot Blot experiment of peptide using 84549-1-RR

  Dot blot analysis was used to confirm the specificity of 84549-1-RR Acetyl-Histone H2B (Lys20) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.

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  ChIP experiment of HeLa using 84549-1-RR

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 3 µg of Acetyl-Histone H2B (Lys20) (84549-1-RR) or 3 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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Applications testées

• Résultats positifs en WB: Trichostatin A treated NIH/3T3 cells,
• Résultats positifs en IF/ICC: cellules HeLa, Trichostatin A treated HeLa cells, Trichostatin A treated NIH/3T3 cells
• Résultats positifs en Dot Blot: Peptide,
• Résultats positifs en ChIP-qPCR: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• DOT BLOT: DOT BLOT : 1:10-1:100
• CHImmunoprécipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84549-1-RR cible Acetyl-Histone H2B (Lys20) dans les applications de WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: histone cluster 2, H2be
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC005827
• Symbole du gène: Histone H2B
• Identification du gène (NCBI): 8349
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

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