Anticorps Recombinant de lapin anti-Acetyl-Histone H3 (Lys23)

Acetyl-Histone H3 (Lys23) Recombinant Antibody for IHC, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IHC, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR

Conjugaison

Non conjugué

CloneNo.

241137E4

N° de cat : 82838-2-PBS

Synonymes

Acetyl Histone, H3 Ac Lys23, H3 Acetyl K23, H3 Acetyl Lys23, H3 Acetyl Lysine23

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Galerie de données de validation

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  IHC staining of mouse testis using 82838-2-RR (same clone as 82838-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82838-2-RR (HIST1H3A antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 82838-2-RR (same clone as 82838-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82838-2-RR (Acetyl-Histone H3 (Lys23) antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.

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  IHC staining of rat testis using 82838-2-RR (same clone as 82838-2-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 82838-2-RR (Acetyl-Histone H3 (Lys23) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82838-2-RR (same clone as 82838-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H3 (Lys23) antibody (82838-2-RR, Clone: 241137E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.

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  Dot Blot experiment of peptide using 82838-2-RR (same clone as 82838-2-PBS)

  Dot blot analysis was used to confirm the specificity of Acetyl-Histone H3 (Lys23) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.

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  ChIP experiment of HeLa using 82838-2-RR (same clone as 82838-2-PBS)

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H3 (Lys23) (82838-2-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.

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Informations sur le produit

82838-2-PBS cible Acetyl-Histone H3 (Lys23) dans les applications de IHC, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: histone cluster 1, H3a
• Numéro d’acquisition GenBank: BC066245
• Symbole du gène: HIST1H3A
• Identification du gène (NCBI): 8350
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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