Anticorps Recombinant de lapin anti-Acetyl-Histone H3 (Lys4)

Acetyl-Histone H3 (Lys4) Recombinant Antibody for WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR

Conjugaison

Non conjugué

CloneNo.

241882E9

N° de cat : 84831-1-RR

Synonymes

Ac Histone H3, Ac Histone H3 (Lys4), Ac Histone H3 Lys4, Ac Histone H3-Lys4, Acetyl Histone

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Galerie de données de validation

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  WB analysis of HeLa using 84831-1-RR

  Trichostatin A treated and untreated HeLa cells were subjected to SDS PAGE followed by western blot with 84831-1-RR (HIST1H3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

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  WB analysis of NIH/3T3 using 84831-1-RR

  Trichostatin A treated and untreated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84831-1-RR (HIST1H3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

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  IF Staining of HeLa using 84831-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84831-1-RR, Clone: 241882E9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HeLa using 84831-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated HeLa cells using Acetyl-Histone H3 (Lys4) antibody (84831-1-RR, Clone: 241882E9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Dot Blot experiment of peptide using 84831-1-RR

  Dot blot analysis was used to confirm the specificity of Acetyl-Histone H3 (Lys4) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

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  ChIP experiment of HeLa using 84831-1-RR

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H3 (Lys4) (84831-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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Applications testées

• Résultats positifs en WB: Trichostatin A treated NIH/3T3 cells, Trichostatin A treated HeLa cells
• Résultats positifs en IF/ICC: Trichostatin A treated HeLa cells, cellules HeLa
• Résultats positifs en Dot Blot: Peptide,
• Résultats positifs en ChIP-qPCR: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• DOT BLOT: DOT BLOT : 1:10-1:100
• CHImmunoprécipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84831-1-RR cible Acetyl-Histone H3 (Lys4) dans les applications de WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: histone cluster 1, H3a
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC066245
• Symbole du gène: HIST1H3A
• Identification du gène (NCBI): 8350
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

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