Anticorps Recombinant de lapin anti-Acetyl-Histone H3 (Lys9)

Acetyl-Histone H3 (Lys9) Recombinant Antibody for WB, IF/ICC, ChIP, Dot Blot, Indirect ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ChIP, Dot Blot, Indirect ELISA, ChIP-qPCR

Conjugaison

Non conjugué

CloneNo.

241596F9

N° de cat : 84543-1-PBS

Synonymes

Acetyl Histone, H3 Ac Lys9, H3 Acetyl K9, H3 Acetyl Lys9, H3 Acetyl Lysine9

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Galerie de données de validation

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  WB analysis of HEK-293 using 84543-1-RR (same clone as 84543-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 84543-1-RR (Acetyl-Histone H3 (Lys9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84543-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 84543-1-RR (same clone as 84543-1-PBS)

  Trichostatin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 84543-1-RR (Acetyl-Histone H3 (Lys9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin Monoclonal antibody (66031-1-Ig) as loading control. This data was developed using the same antibody clone with 84543-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84543-1-RR (same clone as 84543-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84543-1-RR, Clone: 241596F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84543-1-PBS in a different storage buffer formulation.

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  Dot Blot experiment of peptide using 84543-1-RR (same clone as 84543-1-PBS)

  Dot blot analysis was used to confirm the specificity of Acetyl-Histone H3 (Lys9) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 84543-1-PBS in a different storage buffer formulation.

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  ChIP experiment of HeLa using 84543-1-RR (same clone as 84543-1-PBS)

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H3 (Lys9) (84543-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 84543-1-PBS in a different storage buffer formulation.

• 

  ChIP experiment of HeLa using 84543-1-RR (same clone as 84543-1-PBS)

  Chromatin was prepared from HeLa cells, cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H3 (Lys9) (84543-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 84543-1-PBS in a different storage buffer formulation.

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Informations sur le produit

84543-1-PBS cible Acetyl-Histone H3 (Lys9) dans les applications de WB, IF/ICC, ChIP, Dot Blot, Indirect ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: histone cluster 1, H3a
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC066245
• Symbole du gène: HIST1H3A
• Identification du gène (NCBI): 8350
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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