Anticorps Recombinant de lapin anti-Acetyl-Histone H4 (Lys12)

• Phare
• Validé par KD/KO

Acetyl-Histone H4 (Lys12) Recombinant Antibody for WB, IHC, IF/ICC, Dot Blot, ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Dot Blot, ELISA, ChIP-qPCR

Conjugaison

Non conjugué

CloneNo.

1D18

Avis (1)

N° de cat : 83095-1-RR

Synonymes

Ac Histone H4, Ac Histone H4 (Lys12), Ac Histone H4 Lys12, Ac Histone H4-Lys12, Acetyl Histone

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 83095-1-RR

  Untreated and trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Beta Actin Monoclonal antibody (66009-1-Ig) as loading control.

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  WB analysis of HEK-293 using 83095-1-RR

  WB result of Acetyl-Histone H4 (Lys12) antibody (83095-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Acetyl-Histone H4 (Lys12) transfected HEK-293 cells.

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  WB analysis of HSC-T6 using 83095-1-RR

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 83095-1-RR

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse small intestine using 83095-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 83095-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Caco-2 using 83095-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Acetyl-Histone H4 (Lys12) antibody (83095-1-RR, Clone: 1D18 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of Caco-2 using 83095-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Acetyl-Histone H4 (Lys12) antibody (83095-1-RR, Clone: 1D18 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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  Dot Blot experiment of peptide using 83095-1-RR

  Dot blot analysis was used to confirm the specificity of Histone H4AK12ac antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. Column 1: H4AK12ac. Column 2: Unmodified H4AK12. Column 3: H4AK5ac. Column 4: Unmodified H4AK5. Column 5: H4AK8Ac. Column 6: Unmodified H4AK8. Column 7: H4AK16ac. Column 8:Unmodified H4AK16. Column 9: H4AK91ac. Column 10: Unmodified H4K91 Column 11: Blank(PBS).

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  ChIP experiment of HeLa using 83095-1-RR

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H4 (Lys12) (83095-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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Applications testées

• Résultats positifs en WB: Trichostatin A treated NIH/3T3 cells, cellules HEK-293, cellules HSC-T6, cellules NIH/3T3
• Résultats positifs en IHC: tissu d'intestin grêle de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules Caco-2,
• Résultats positifs en Dot Blot: Peptide,
• Résultats positifs en ChIP-qPCR: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600
• DOT BLOT: DOT BLOT : 1:10-1:100
• CHImmunoprécipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83095-1-RR cible Acetyl-Histone H4 (Lys12) dans les applications de WB, IHC, IF/ICC, Dot Blot, ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: histone cluster 1, H4a
• Poids moléculaire observé: 12 kDa
• Numéro d’acquisition GenBank: BC069654
• Symbole du gène: HIST1H4A
• Identification du gène (NCBI): 8359
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Histone H4 is a 103 amino acid protein, which belongs to the histone H4 family. Histone H4 localizes in the nucleus and is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Acetylation of histone H4 is necessary for chromatin decompaction during DNA replication.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sree Rama Chaitanya (Verified Customer) (07-21-2025)

I have tested this antibody to evaluate H4K12ac levels in mammalian cells, and it works well for WB. Highly recommend!

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF10A

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