Anticorps Polyclonal de lapin anti-Aconitase 2

Aconitase 2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11134-1-PBS

Synonymes

ACO2, ACO 2, EC:4.2.1.3, Citrate hydro-lyase, Aconitase2

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Galerie de données de validation

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  WB analysis using 11134-1-AP (same clone as 11134-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11134-1-AP (Aconitase 2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11134-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 11134-1-AP (same clone as 11134-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 11134-1-AP (Aconitase 2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11134-1-PBS in a different storage buffer formulation.

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  WB analysis of human skeletal muscle using 11134-1-AP (same clone as 11134-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11134-1-AP (Aconitase 2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11134-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 11134-1-AP (same clone as 11134-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11134-1-AP (Aconitase 2 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11134-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 11134-1-AP (same clone as 11134-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11134-1-AP (Aconitase 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11134-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 11134-1-AP (same clone as 11134-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11134-1-AP (Aconitase 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11134-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 11134-1-AP (same clone as 11134-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11134-1-AP (Aconitase 2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11134-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 11134-1-AP (same clone as 11134-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11134-1-AP (Aconitase 2 antibody) at dilution of 1:50 (under 25x lens). This data was developed using the same antibody clone with 11134-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11134-1-AP (same clone as 11134-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Aconitase 2 antibody (11134-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11134-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11134-1-PBS cible Aconitase 2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Aconitase 2 Protéine recombinante Ag1590
• Nom complet: aconitase 2, mitochondrial
• Masse moléculaire calculée: 85 kDa
• Poids moléculaire observé: 85 kDa
• Numéro d’acquisition GenBank: BC014092
• Symbole du gène: ACO2
• Identification du gène (NCBI): 50
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ACO2(aconitate hydratase, mitochondrial) is also named as citrate hydro-lyase and belongs to the aconitase/IPM isomerase family. It plays a key function in cellular energy production, and loss of its activity has a major impact on cellular and organismal survival. Western blot shows two bands of 83 kDa and 40 kDa. The 40 kDa fragment decreases with age and oxidative stress, whereas other fragmentation products with molecular weights between 40 and 83 kDa increased with age and MnSOD(mitochondrial manganese superoxide dismutase) deficiency(PMID:12459471). Defects in ACO2 are the cause of infantile cerebellar-retinal degeneration (ICRD).

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