Anticorps Polyclonal de lapin anti-ADSL

ADSL Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15264-1-PBS

Synonymes

EC:4.3.2.2, ASL, ASASE, AMPS

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Galerie de données de validation

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  WB analysis using 15264-1-AP (same clone as 15264-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15264-1-AP (Adenylosuccinate lyase antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15264-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 15264-1-AP (same clone as 15264-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15264-1-AP (Adenylosuccinate lyase antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15264-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 15264-1-AP (same clone as 15264-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15264-1-AP (Adenylosuccinate lyase antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15264-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15264-1-AP (same clone as 15264-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15264-1-AP (Adenylosuccinate lyase antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15264-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15264-1-AP (same clone as 15264-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15264-1-AP (Adenylosuccinate lyase antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15264-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 15264-1-AP (same clone as 15264-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Adenylosuccinate lyase antibody (15264-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 15264-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 15264-1-AP (same clone as 15264-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Adenylosuccinate lyase Polyclonal antibody (15264-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 15264-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15264-1-PBS cible ADSL dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ADSL Protéine recombinante Ag7332
• Nom complet: adenylosuccinate lyase
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC000253
• Symbole du gène: ADSL
• Identification du gène (NCBI): 158
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ADSL(adenylosuccinate lyase) is also named as AMPS, ASase, ASL and belongs to the lyase 1 family. It is an enzyme involved in 2 pathways of purine nucleotide metabolism and catalyzes cleavage of succinyl groups to yield fumarate(PMID:18524658). Defects in ADSL are the cause of adenylosuccinase deficiency (ADSL deficiency). In humans, mutations in ADSL lead to an inborn error of metabolism originally characterized by developmental delay, often with autistic features(PMID:20884265)..The ADSL enzymatic activity is reduced in lymphocytes and red blood cells of the patient with severe psychomotor retardation(PMID:9545543). It has 2 isoforms produced by alternative splicing.

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