Anticorps Recombinant de lapin anti-Adiponectin

Adiponectin Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

230258C5

N° de cat : 83070-3-PBS

Synonymes

Adipoq, apM1, adipo, Acrp30, 230258C5

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP00039-3

  Sandwich ELISA standard curve of MP00039-3, MOUSE Adiponectin Recombinant Matched Antibody Pair - PBS only. 83070-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0262. 83070-3-PBS was HRP conjugated as the detection antibody. Range:15.6-1000pg/mL.

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  Sample test of MP00039-3

  Serum of sixteen mice was measured. The Adiponectin concentration of detected samples was determined to be 7.30 ug/mL with a range of 4.72-13.56 ug/mL.

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  Sample test of MP00039-3

  lungs from one mouse were chopped into 1-2 mm pieces and seeded into approximately 30 mL of media containing RPMI, 10% fetal bovine serum, 50 μM β-mercaptoethanol, and L-glutamine.The mean Adiponectin concentration was determined to be 4.4 ng/mL in lung cell culture supernatant.

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  Sample test of MP00039-3

  Organs from 2 mice were rinsed with PBS to remove excess blood, chopped into 1-2 mm pieces, homogenized in 5-10 mL of PBS in a tissue homogenizer, and stored at ≤ -80℃ 5 min. After two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000 x g to remove particulate. The mean Adiponectin concentration was determined to be 130.21 ng/mL in spleen homogenates, 745.9 ng/mL in fat tissue homogenates.

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Informations sur le produit

83070-3-PBS cible Adiponectin dans les applications de Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: adiponectin, C1Q and collagen domain containing
• Masse moléculaire calculée: 27kd
• Numéro d’acquisition GenBank: NM_009605
• Symbole du gène: Adiponectin
• Identification du gène (NCBI): 11450
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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