Anticorps Recombinant de lapin anti-Adiponectin

Adiponectin Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240392D6

N° de cat : 83525-2-RR

Synonymes

Adipoq, Acrp30, Acdc, 240392D6

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Galerie de données de validation

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  WB analysis of mouse adipose using 83525-2-RR

  mouse adipose tissue were subjected to SDS PAGE followed by western blot with 83525-2-RR (Adipoq antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat adipose using 83525-2-RR

  rat adipose tissue were subjected to SDS PAGE followed by western blot with 83525-2-RR (Adiponectin antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 83525-2-RR

  rat heart tissue were subjected to SDS PAGE followed by western blot with 83525-2-RR (Adipoq antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83525-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83525-2-RR against Rat Adiponectin were performed. The affinity constant is 0.149 nM.

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Applications testées

• Résultats positifs en WB: rat adipose tissue, tissu adipeux de souris, tissu cardiaque de rat

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:15000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83525-2-RR cible Adiponectin dans les applications de WB, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: adiponectin, C1Q and collagen domain containing
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 26-28 kDa
• Numéro d’acquisition GenBank: NM_144744.3
• Symbole du gène: Adiponectin
• Identification du gène (NCBI): 246253
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number of metabolic processes, including improving INS sensitivity and anti-inflammatory activity. The role of AdipoQ in reproduction is not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.

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