Anticorps Recombinant de lapin anti-Afp

Afp Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240232C7

N° de cat : 83367-2-PBS

Synonymes

240232C7, alpha fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein

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Galerie de données de validation

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  WB analysis of mouse placenta using 83367-2-RR (same clone as 83367-2-PBS)

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 83367-2-RR (Afp antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83367-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00395-4

  Sandwich ELISA standard curve of MP00395-4, Mouse AFP Monoclonal Matched Antibody Pair - PBS only. 83367-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0670. 83367-2-PBS was HRP conjugated as the detection antibody. Range: 78.125-5000 pg/mL.

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  Sample test of MP00395-4

  Serum of sixteen individual healthy mice were measured. The mouse AFP concentration of detected samples was determined to be113.4 ng/mL with a range of 66.5-245.7 ng/mL.

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  Affinity and Kinetic Characterization of 83367-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83367-2-RR against Mouse Afp were performed. The affinity constant is 1.69 nM.

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Informations sur le produit

83367-2-PBS cible Afp dans les applications de WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Afp Protéine recombinante Eg0670
• Nom complet: alpha fetoprotein
• Masse moléculaire calculée: 67KD
• Poids moléculaire observé: 67-72 kDa
• Numéro d’acquisition GenBank: NM_007423.4
• Symbole du gène: Afp
• Identification du gène (NCBI): 11576
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Alpha-fetoprotein (AFP) is one of the earliest proteins to be synthesised by the embryonic liver. The synthesis of AFP decreases dramatically after birth and only trace amounts are expressed in the adult liver. High serum AFP concentrations are also found in connection with primary liver cancer and teratocarcinoma.

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