Anticorps Recombinant de lapin anti-YTHDF1

YTHDF1 Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241445B10

N° de cat : 80876-2-PBS

Synonymes

241445B10, C20orf21, DACA-1, DF1, YTH domain-containing family protein 1

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Galerie de données de validation

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  WB analysis using 80876-2-RR (same clone as 80876-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80876-2-RR (Ag11509 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80876-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 80876-2-RR (same clone as 80876-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80876-2-RR (YTHDF1 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80876-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 80876-2-RR (same clone as 80876-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using YTHDF1 antibody (80876-2-RR, Clone: 241445B10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80876-2-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 80876-2-RR (same clone as 80876-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using YTHDF1 antibody (80876-2-RR, Clone: 241445B10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80876-2-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 80876-2-RR (same clone as 80876-2-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug YTHDF1 Recombinant antibody (80876-2-RR, Clone:241445B10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80876-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80876-2-RR

  Biolayer interferometry (BLl) kinetic assays of 80876-2-RR against Human YTHDF1 were performed. The affinity constant is 7.23 nM.

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Informations sur le produit

80876-2-PBS cible YTHDF1 dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: YTHDF1 Protéine recombinante Ag11509
• Nom complet: YTH domain family, member 1
• Masse moléculaire calculée: 559 aa, 61 kDa
• Poids moléculaire observé: 60-65 kDa
• Numéro d’acquisition GenBank: BC050284
• Symbole du gène: YTHDF1
• Identification du gène (NCBI): 54915
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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