Anticorps Monoclonal anti-AlaRS

• Phare
• Validé par KD/KO

AlaRS Monoclonal Antibody for WB, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C8H3

N° de cat : 67909-1-PBS

Synonymes

AARS, 1C8H3, AARS1, Alanine--tRNA ligase, cytoplasmic, Alanyl-tRNA synthetase

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Galerie de données de validation

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  WB analysis using 67909-1-Ig (same clone as 67909-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67909-1-Ig (AlaRS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 67909-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67909-1-Ig (same clone as 67909-1-PBS)

  WB result of AlaRS antibody (67909-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AlaRS transfected HeLa cells. This data was developed using the same antibody clone with 67909-1-PBS in a different storage buffer formulation.

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  WB analysis using 67909-1-Ig (same clone as 67909-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67909-1-Ig (AARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67909-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 67909-1-Ig (same clone as 67909-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using AlaRS antibody (67909-1-Ig, Clone: 1C8H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67909-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 67909-1-Ig (same clone as 67909-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using AlaRS antibody (67909-1-Ig, Clone: 1C8H3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67909-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67909-1-Ig (same clone as 67909-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AlaRS antibody (67909-1-Ig, Clone: 1C8H3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67909-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67909-1-Ig (same clone as 67909-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using AlaRS antibody (67909-1-Ig, Clone: 1C8H3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67909-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67909-1-PBS cible AlaRS dans les applications de WB, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AlaRS Protéine recombinante Ag11151
• Nom complet: alanyl-tRNA synthetase
• Masse moléculaire calculée: 968 aa, 107 kDa
• Poids moléculaire observé: 107 kDa
• Numéro d’acquisition GenBank: BC011451
• Symbole du gène: AlaRS
• Identification du gène (NCBI): 16
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AARS(alanyl-tRNA synthetase) is also named as AlaRS(alanine tRNA ligase 1, cytoplasmic), renal carcinoma antigen NY-REN-42 and belongs to the class-II aminoacyl-tRNA synthetase family. It can interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. AARS consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs(PMID:19661429).

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