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Anticorps Monoclonal anti-AlaRS

AlaRS Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite®555 Fluorescent Dye

CloneNo.

1C8H3

N° de cat : CL555-67909

Synonymes

AARS, Alanine tRNA ligase, alanyl tRNA synthetase, AlaRS

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®555-conjugated AlaRS Monoclonal antibody (CL555-67909)

    IF Staining of HeLa using CL555-67909

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®555 AlaRS antibody (CL555-67909, Clone: 1C8H3 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of K-562 cells using CoraLite®555-conjugated AlaRS Monoclonal antibody (CL555-67909)

    FC experiment of K-562 using CL555-67909

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human AlaRS (CL555-67909, Clone:1C8H3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en IFcellules HeLa,
Résultats positifs en cytométriecellules K-562

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-67909 cible AlaRS dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène AlaRS Protéine recombinante Ag11151
Nom complet alanyl-tRNA synthetase
Masse moléculaire calculée 968 aa, 107 kDa
Poids moléculaire observé107 kDa
Numéro d’acquisition GenBankBC011451
Symbole du gène AARS
Identification du gène (NCBI) 16
Conjugaison CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AARS(alanyl-tRNA synthetase) is also named as AlaRS(alanine tRNA ligase 1, cytoplasmic), renal carcinoma antigen NY-REN-42 and belongs to the class-II aminoacyl-tRNA synthetase family. It can interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. AARS consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs(PMID:19661429).

Protocole

Product Specific Protocols
IF protocol for CL555 AlaRS antibody CL555-67909Download protocol
FC protocol for CL555 AlaRS antibody CL555-67909Download protocol
Standard Protocols
Click here to view our Standard Protocols