Anticorps Monoclonal anti-Albumin

Albumin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat

Applications

WB, IHC, IF/ICC, IF-P, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

4A1C11

N° de cat : 66051-1-PBS

Synonymes

ALB, 4A1C11, HSA, Serum albumin

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Galerie de données de validation

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  WB analysis of human plasma using 66051-1-Ig (same clone as 66051-1-PBS)

  human plasma (diluted 5000 fold) was subjected to SDS PAGE followed by western blot with 66051-1-Ig (Albumin Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 66051-1-Ig (same clone as 66051-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 66051-1-Ig (ALB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50031-1

  Sandwich ELISA standard curve of MP50031-1, Albumin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66051-2-PBS. Detection antibody: 66051-1-PBS. Standard: Ag9885. Range: 50-3200 ng/mL.

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  Sample test of MP50031-1

  Human cerebrospinal fluid (CSF) of one individual human donors was measured. The Albumin concentration of detected samples was determined to be 29.50 μg/mL.

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  Sample test of MP50031-1

  Serum of four individual healthy human donors was measured. The Albumin concentration of detected samples was determined to be 29.65 mg/mL with a range of 11.40-48.00 mg/mL.

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  Sample test of MP50031-1

  Urine of four individual healthy human donors was measured. The Albumin concentration of detected samples was determined to be 1,036.00 ng/mL with a range of 560.00-1,616.00 ng/mL.

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  Sample test of MP50031-1

  Saliva of two individual healthy human donors was measured. The Albumin concentrations of detected samples were determined to be 32.00 μg/mL and 18.60 μg/mL, respectively.

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  Sample test of MP50031-1

  Human milk of two individual healthy human donors was measured. The Albumin concentrations of detected samples were determined to be 106.00 μg/mL and 116.00 μg/mL, respectively.

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  IP experiment of HepG2 using 66051-1-Ig (same clone as 66051-1-PBS)

  IP result of anti-Albumin (IP:66051-1-Ig, 4ug; Detection:66051-1-Ig 1:1000) with HepG2 cells lysate 1720 ug. This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66051-1-Ig (same clone as 66051-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66051-1-Ig(ALB antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66051-1-Ig (same clone as 66051-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66051-1-Ig(ALB antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66051-1-Ig (same clone as 66051-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66051-1-Ig (Albumin antibody) at dilution of 1:64000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 66051-1-Ig (same clone as 66051-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Albumin antibody (66051-1-Ig, Clone: 4A1C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 66051-1-Ig (same clone as 66051-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Albumin antibody (66051-1-Ig, Clone: 4A1C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66051-1-Ig (same clone as 66051-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Albumin antibody (66051-1-Ig, Clone: 4A1C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66051-1-Ig (same clone as 66051-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Albumin antibody (66051-1-Ig, Clone: 4A1C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 66051-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 66051-1-Ig against Human Albumin were performed. The affinity constant is 0.574 nM

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Informations sur le produit

66051-1-PBS cible Albumin dans les applications de WB, IHC, IF/ICC, IF-P, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, porc, rat

• Réactivité: Humain, porc, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Albumin Protéine recombinante Ag9885
• Nom complet: albumin
• Masse moléculaire calculée: 609 aa, 69 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC034023
• Symbole du gène: Albumin
• Identification du gène (NCBI): 213
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071) In additon, glycated serum albumin is also a potential diabetes biomarker.

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