Anticorps Polyclonal de lapin anti-Alpha-1-antitrypsin

Alpha-1-antitrypsin Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25876-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 25876-1-AP (same clone as 25876-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25876-1-AP (Alpha-1-antitrypsin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25876-1-PBS in a different storage buffer formulation.

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  IP experiment of human plasma using 25876-1-AP (same clone as 25876-1-PBS)

  IP result of anti-Alpha-1-antitrypsin (IP:25876-1-AP, 4ug; Detection:25876-1-AP 1:3000) with human plasma lysate 1600 ug. This data was developed using the same antibody clone with 25876-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse placenta using 25876-1-AP (same clone as 25876-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 25876-1-AP (Alpha-1-antitrypsin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25876-1-PBS in a different storage buffer formulation.

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Informations sur le produit

25876-1-PBS cible Alpha-1-antitrypsin dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Alpha-1-antitrypsin Protéine recombinante Ag22631
• Nom complet: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
• Poids moléculaire observé: 47-50 kDa
• Numéro d’acquisition GenBank: BC011991
• Symbole du gène: Alpha 1-Antitrypsin
• Identification du gène (NCBI): 5265
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SERPINA1 is the gene for a protein called alpha-1-antitrypsin (AAT), which is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. AAT is a glycoprotein synthesized primarily by hepatocytes, with smaller amountssynthesized by intestinal epithelial cells, neutrophils, pulmonary alveolar cells and macrophages. AAT is the most abundant, endogenous serine protease inhibitor in blood circulation and it has been implicated in regulating vital fluid phase biological events such as blood coagulation, fibrinolysis, complement activation, apoptosis, reproduction, tumor progression and inflammatory response. The primary function of AAT is thought to be the inactivation of neutrophil elastase and other endogenous serine proteases. Defects in SERPINA1 can cause emphysema or liver disease.

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