Anticorps Recombinant de lapin anti-Alpha-1-microglobulin

Alpha-1-microglobulin Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240751G7

N° de cat : 83678-3-PBS

Synonymes

AMBP, Alpha 1-microglobulin, Alpha 1 microglycoprotein, Alpha 1 microglobulin, A1M

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Galerie de données de validation

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  WB analysis of human plasma using 83678-3-RR (same clone as 83678-3-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 83678-3-RR (Alpha-1-microglobulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83678-3-PBS in a different storage buffer formulation.

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  WB analysis of human urine using 83678-3-RR (same clone as 83678-3-PBS)

  human urine sample were subjected to SDS PAGE followed by western blot with 83678-3-RR (Alpha-1-microglobulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83678-3-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 83678-3-RR (same clone as 83678-3-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Alpha 1 microglobulin Recombinant antibody (83678-3-RR, Clone:240751G7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83678-3-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83678-3-RR (same clone as 83678-3-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Alpha 1 microglobulin Recombinant antibody (83678-3-RR, Clone:240751G7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83678-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83678-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83678-3-RR against Human Alpha 1 microglobulin were performed. The affinity constant is 6.59 nM.

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Informations sur le produit

83678-3-PBS cible Alpha-1-microglobulin dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: alpha-1-microglobulin/bikunin precursor
• Masse moléculaire calculée: 39kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: NM_001633.4
• Symbole du gène: Alpha 1 microglobulin
• Identification du gène (NCBI): 259
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Alpha 1 microglobulin, also known as HI30, is a 27-30 kDa glycoprotein, present in various body fluids. It belongs to the lipocalin superfamily of hydrophobic ligand-binding proteins forming an internal ligand-binding pocket. The protein acts as a mediator of bacterial adhesion to polymer surfaces and is involved in inhibiting renal lithogenesis.

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