Anticorps Polyclonal de lapin anti-Alpha 1B-Glycoprotein

Alpha 1B-Glycoprotein Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14181-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of human plasma using 14181-1-AP (same clone as 14181-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14181-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 14181-1-AP (same clone as 14181-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14181-1-PBS in a different storage buffer formulation.

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  WB analysis of BxPC-3 using 14181-1-AP (same clone as 14181-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14181-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 14181-1-AP (same clone as 14181-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14181-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 14181-1-AP (same clone as 14181-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14181-1-AP (Alpha 1B-Glycoprotein antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14181-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 14181-1-AP (same clone as 14181-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14181-1-AP (Alpha 1B-Glycoprotein antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14181-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14181-1-AP (same clone as 14181-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:25 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14181-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14181-1-PBS cible Alpha 1B-Glycoprotein dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Alpha 1B-Glycoprotein Protéine recombinante Ag5414
• Nom complet: alpha-1-B glycoprotein
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC035719
• Symbole du gène: A1BG
• Identification du gène (NCBI): 1
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Alpha-1B-glycoprotein (A1BG) is a plasma protein and is a member of the immunoglobulin superfamily. A1BG contains 474 amino acids and has 5 intrachain disulfide bonds. It has been reported that cysteine-rich secretory protein 3 (CRISP-3) is a specific and high-affinity ligand of A1BG. A1BG-CRISP-3 complex displays a similar function in protecting the circulation from a potentially harmful effect of free CRISP3 (PMID: 15461460).

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