Anticorps Polyclonal de lapin anti-Alpha Actinin

• Phare
• Validé par KD/KO

Alpha Actinin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11313-2-PBS

Synonymes

ACTN1, Alpha actinin 1, actin, actinin, alpha 1, ACTN 1

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Galerie de données de validation

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  WB analysis using 11313-2-AP (same clone as 11313-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11313-2-AP (Alpha Actinin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11313-2-AP (same clone as 11313-2-PBS)

  WB result of Alpha Actinin antibody (11313-2-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alpha Actinin transfected HeLa cells. This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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  WB analysis of rat heart using 11313-2-AP (same clone as 11313-2-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11313-2-AP (alpha Actinin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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  WB analysis of mouse spleen using 11313-2-AP (same clone as 11313-2-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11313-2-AP (alpha Actinin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 11313-2-AP (same clone as 11313-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11313-2-AP (alpha Actinin antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 11313-2-AP (same clone as 11313-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11313-2-AP (alpha Actinin antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 11313-2-AP (same clone as 11313-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11313-2-AP (Alpha Actinin antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 11313-2-AP (same clone as 11313-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 11313-2-AP (alpha Actinin antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 66219-1-Ig (N-cadherin) in red. This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 11313-2-AP (same clone as 11313-2-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed mouse heart tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse skeletal muscle using 11313-2-AP (same clone as 11313-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse skeletal muscle using 11313-2-AP (same clone as 11313-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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  IF Staining of rat heart using 11313-2-AP (same clone as 11313-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

• 

  IF Staining of rat heart using 11313-2-AP (same clone as 11313-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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  IF Staining of HSkMC using 11313-2-AP (same clone as 11313-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HSkMC cells using Alpha Actinin antibody (11313-2-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 11313-2-AP (same clone as 11313-2-PBS)

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Alpha Actinin (11313-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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Informations sur le produit

11313-2-PBS cible Alpha Actinin dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Alpha Actinin Protéine recombinante Ag1859
• Nom complet: actinin, alpha 1
• Masse moléculaire calculée: 103 kDa
• Poids moléculaire observé: 100-105 kDa
• Numéro d’acquisition GenBank: BC015766
• Symbole du gène: Alpha Actinin
• Identification du gène (NCBI): 87
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.

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