Anticorps Monoclonal anti-Alpha Actinin

Alpha Actinin Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E2E1

N° de cat : 66895-1-PBS

Synonymes

ACTN1, Alpha actinin 1, 1E2E1, actin, actinin, alpha 1

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Galerie de données de validation

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  WB analysis using 66895-1-Ig (same clone as 66895-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66895-1-Ig (a-Actinin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

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  WB analysis using 66895-1-Ig (same clone as 66895-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66895-1-Ig (Alpha Actinin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66895-1-Ig (same clone as 66895-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66895-1-Ig (Alpha Actinin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66895-1-Ig (same clone as 66895-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66895-1-Ig (a-Actinin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 66895-1-Ig (same clone as 66895-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66895-1-Ig (alpha Actinin antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 66895-1-Ig (same clone as 66895-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66895-1-Ig (alpha Actinin antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 66895-1-Ig (same clone as 66895-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Alpha Actinin antibody (66895-1-Ig, Clone: 1E2E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 66895-1-Ig (same clone as 66895-1-PBS)

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Alpha Actinin (66895-1-Ig, Clone:1E2E1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66895-1-PBS cible Alpha Actinin dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Alpha Actinin Protéine recombinante Ag28383
• Nom complet: actinin, alpha 1
• Masse moléculaire calculée: 103 kDa
• Poids moléculaire observé: 100-105 kDa
• Numéro d’acquisition GenBank: BC015766
• Symbole du gène: Alpha Actinin
• Identification du gène (NCBI): 87
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.

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