Anticorps Polyclonal de lapin anti-Alpha Adaptin

Alpha Adaptin Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11401-1-PBS

Synonymes

AP2A1, Alpha1 adaptin, Alpha adaptin A, ADTAA, Adaptor-related protein complex 2 subunit alpha-1

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Galerie de données de validation

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  WB analysis of HEK-293 using 11401-1-AP (same clone as 11401-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11401-1-AP (alpha Adaptin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11401-1-PBS in a different storage buffer formulation.

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  WB analysis of human spleen using 11401-1-AP (same clone as 11401-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11401-1-AP (alpha Adaptin antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11401-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 11401-1-AP (same clone as 11401-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 11401-1-AP (alpha Adaptin antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11401-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 11401-1-AP (same clone as 11401-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11401-1-AP (alpha Adaptin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11401-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11401-1-AP (same clone as 11401-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Adaptin antibody (11401-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11401-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11401-1-PBS cible Alpha Adaptin dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Alpha Adaptin Protéine recombinante Ag1927
• Nom complet: adaptor-related protein complex 2, alpha 1 subunit
• Masse moléculaire calculée: 977 aa, 108 kDa
• Poids moléculaire observé: 108 kDa
• Numéro d’acquisition GenBank: BC014214
• Symbole du gène: AP2A1
• Identification du gène (NCBI): 160
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AP2A1 encodes adaptor protein complex 2 (AP-2) subunit alpha-1, which is a 100 kDa coated vesicle protein. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in the cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 is involved in clathrin-dependent endocytosis.

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