Anticorps Monoclonal anti-Alpha 1 antichymotrypsin

Alpha 1 antichymotrypsin Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3F2F10

N° de cat : 66078-1-PBS

Synonymes

Alpha Antichymotrypsin, SERPINA3, ACT, AACT, A-1-Antichymotrypsin

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Galerie de données de validation

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  WB analysis of human plasma using 66078-1-Ig (same clone as 66078-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66078-1-Ig (Alpha Antichymotrypsin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66078-1-PBS in a different storage buffer formulation.

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  WB analysis of human plasma using 66078-1-Ig (same clone as 66078-1-PBS)

  Untreated and PNGase F-treated lysates of human plasma were subjected to SDS PAGE followed by western blot with 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. Loading amount:0.7 μL crude plasma was lysised for each lane. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66078-1-PBS in a different storage buffer formulation.

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  WB analysis of human colon using 66078-1-Ig (same clone as 66078-1-PBS)

  Untreated and PNGase F-treated lysates of human colon tissue (25μg/lane) were subjected to SDS PAGE followed by western blot with 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66078-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66078-1-Ig (same clone as 66078-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66078-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66078-1-Ig (same clone as 66078-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66078-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66078-1-Ig (same clone as 66078-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66078-1-Ig(alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66078-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66078-1-Ig (same clone as 66078-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66078-1-Ig(alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66078-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66078-1-Ig (same clone as 66078-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66078-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66078-1-PBS cible Alpha 1 antichymotrypsin dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Alpha 1 antichymotrypsin Protéine recombinante Ag2830
• Nom complet: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3
• Masse moléculaire calculée: 448 aa, 48 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC010530
• Symbole du gène: Alpha 1 Antichymotrypsin
• Identification du gène (NCBI): 12
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Human SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a member of the serine protease inhibitor class, and is a plasma alpha globulin glycoprotein which increases in the blood during the inflammatory process. SerpinA3, is also an inhibitor of neutrophil cathepsin G, mast cell chymases and pancreatic chymotrypsin. SerpinA3 is produced primarily in the liver, and is identified as an acute-phase inflammatory protein. SerpinA3 deficiency has been associated with liver disease. SerpinA3 has also been implicated in the pathology of a number of devastating human diseases including chronic obstructive pulmonary disease (COPD), Parkinson's disease (PD), Alzheimer's disease (AD), Stroke, Cystic Fibrosis, Cerebral Haemorrhage and Multiple System Atrophy.

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