Anticorps Polyclonal de lapin anti-Alpha B Crystallin

• Phare
• Validé par KD/KO

Alpha B Crystallin Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15808-1-PBS

Synonymes

CRYAB, Alpha crystallin B chain, Alpha(B) crystallin, Alpha(B)-crystallin, Alpha-crystallin B chain

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Galerie de données de validation

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  WB analysis using 15808-1-AP (same clone as 15808-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15808-1-AP (Alpha B Crystallin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15808-1-PBS in a different storage buffer formulation.

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  WB analysis using 15808-1-AP (same clone as 15808-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15808-1-AP (Alpha B Crystallin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15808-1-PBS in a different storage buffer formulation.

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  IP experiment of U2OS using 15808-1-AP (same clone as 15808-1-PBS)

  IP result of anti-Alpha B Crystallin (IP:15808-1-AP, 4ug; Detection:15808-1-AP 1:500) with U2OS cells lysate 1800 ug. This data was developed using the same antibody clone with 15808-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 15808-1-AP (same clone as 15808-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15808-1-AP (Alpha B Crystallin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15808-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 15808-1-AP (same clone as 15808-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using Alpha B Crystallin antibody (15808-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 15808-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15808-1-PBS cible Alpha B Crystallin dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Alpha B Crystallin Protéine recombinante Ag8543
• Nom complet: crystallin, alpha B
• Masse moléculaire calculée: 175 aa, 20 kDa
• Poids moléculaire observé: 20-22 kDa
• Numéro d’acquisition GenBank: BC007008
• Symbole du gène: Alpha B Crystallin
• Identification du gène (NCBI): 1410
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Alpha B-crystallin, encoded by CRYAB gene, is multifunctional, serving as both a major structural protein in the lens and a small heat-shock protein in other tissues in mammals. Alpha B-crystallin may contribute to the transparency and refractive index of the lens. Single nucleotide polymorphisms (SNPs) in the promoter region of CRYAB gene have been associated with in multiple sclerosis. Mutations in the CRYAB gene cause distinct clinical phenotypes including isolated posterior polar cataract, myofibrillar myopathy, cardiomyopathy, or a multisystemic disorder combining all these features. Impairment of alpha-B crystallin dimerization may be relevant to the pathogenesis of these disorders.

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