Anticorps Monoclonal anti-Alpha Synuclein

Alpha Synuclein Monoclonal Antibody for WB, IHC, IF-P, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B10E9

N° de cat : 66412-1-PBS

Synonymes

1, a-synuclein, SNCA, 1B10E9, Alpha-synuclein

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Galerie de données de validation

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  WB analysis of mouse brain using 66412-1-Ig (same clone as 66412-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66412-1-Ig (a-Synuclein Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 66412-1-Ig (same clone as 66412-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66412-1-Ig (a-Synuclein Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50043-1

  Sandwich ELISA standard curve of MP50043-1, a-Synuclein Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60285-1-PBS. Detection antibody: 66412-1-PBS. Standard: Ag1285. Range: 0.313-20 ng/mL.

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  IHC staining of mouse cerebellum using 66412-1-Ig (same clone as 66412-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66412-1-Ig (alpha-Synuclein antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 66412-1-Ig (same clone as 66412-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66412-1-Ig (alpha-Synuclein antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 66412-1-Ig (same clone as 66412-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66412-1-Ig (a-Synuclein antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66412-1-Ig (same clone as 66412-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66412-1-Ig (alpha-Synuclein antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66412-1-Ig (same clone as 66412-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66412-1-Ig (alpha-Synuclein antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse cerebellum using 66412-1-Ig (same clone as 66412-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using Alpha Synuclein antibody (66412-1-Ig, Clone: 1B10E9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse cerebellum using 66412-1-Ig (same clone as 66412-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using Alpha Synuclein antibody (66412-1-Ig, Clone: 1B10E9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 66412-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 66412-1-Ig against Human SNCA were performed. The affinity constant is 0.388 nM.

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Informations sur le produit

66412-1-PBS cible Alpha Synuclein dans les applications de WB, IHC, IF-P, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Alpha Synuclein Protéine recombinante Ag1285
• Nom complet: synuclein, alpha (non A4 component of amyloid precursor)
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 18-19 kDa
• Numéro d’acquisition GenBank: BC013293
• Symbole du gène: Alpha Synuclein
• Identification du gène (NCBI): 6622
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Alpha Synuclein (α-syn) is a 14-19 kDa phosphoprotein that is primarily localize to the presynaptic terminals of mature neurons, where it is involved in synaptic function and plasticity. α-syn has drawn intense interest ever since the late 1990s, when the first α-synuclein missense mutation was identified as a cause of familial Parkinson's disease (PD). Aggregated and hyper-phosphorylated forms of α-syn protein are the pathological hallmark of Lewy body disease, which includes Parkinson's disease (PD), diffuse Lewy body disease (DLBD), and Lewy body variant of Alzheimer's disease (LBV). This antibody can recognize all the isoforms of α-syn.

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