Anticorps Monoclonal anti-Alpha Tubulin

• Phare
• Validé par KD/KO

Alpha Tubulin Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

canin, Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

CloneNo.

1E4C11

N° de cat : 66031-1-PBS

Synonymes

TUBA1B, Tubulin alpha 1B chain, 1E4C11, Alpha-tubulin ubiquitous, EC:3.6.5.-

---

Galerie de données de validation

• 

  WB analysis using 66031-1-Ig (same clone as 66031-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66031-1-Ig (Alpha Tubulin antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66031-1-Ig (same clone as 66031-1-PBS)

  WB result of Alpha Tubulin antibody (66031-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alpha Tubulin transfected HeLa cells. This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 66031-1-Ig (same clone as 66031-1-PBS)

  IP result of anti-Alpha Tubulin (IP:66031-1-Ig, 5ug; Detection:11224-1-AP 1:1000) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66031-1-Ig (same clone as 66031-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66031-1-Ig (alpha Tubulin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66031-1-Ig (same clone as 66031-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66031-1-Ig (Alpha Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66031-1-Ig (same clone as 66031-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66031-1-Ig (Alpha Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66031-1-Ig (same clone as 66031-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66031-1-Ig (alpha Tubulin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66031-1-Ig (same clone as 66031-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 66031-1-Ig(alpha Tubulin antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66031-1-Ig (same clone as 66031-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11 ) at dilution of 1:1000 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Histone H1.2 antibody (19649-1-AP, green). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66031-1-Ig (same clone as 66031-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66031-1-Ig(alpha Tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 66031-1-Ig (same clone as 66031-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11 ) at dilution of 1:1000 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), ZO-1 antibody (21773-1-AP, green). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 66031-1-Ig (same clone as 66031-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ZO-1 antibody (21773-1-AP, red). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66031-1-Ig (same clone as 66031-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 66031-1-Ig (alpha Tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 66031-1-Ig (same clone as 66031-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Alpha Tubulin (66031-1-Ig, Clone:1E4C11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66031-1-PBS cible Alpha Tubulin dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Alpha Tubulin Protéine recombinante Ag18034
• Nom complet: tubulin, alpha 1b
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC009314
• Symbole du gène: Alpha Tubulin
• Identification du gène (NCBI): 10376
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

What is the function of alpha tubulin?

Alpha-tubulin belongs to a large superfamily of tubulin proteins. There are a number of different subtypes that have a molecular weight of ~50kDa and are able to bind to beta-tubulin, forming a heterodimer that polymerises to microtubules as part of the cytoskeleton. These maintain cell structure, provide platforms for intracellular transport and are also involved in cell division.

 Where is alpha-tubulin expressed?

Alpha tubulin is highly conserved and is present in nearly all eukaryotic cells as one of the building blocks of microtubules. The ubiquitous nature of this protein has led to its common use as a control protein for many tissue types as well as highlighting the structure of the cytoskeleton. 

What are the post-translational modifications of alpha tubulin?

The function and properties of microtubules are drastically affected by the post-translational modifications undergone by tubulin, which may occur to the tubulin dimer directly or to the polymerised mictotubule. For example, the first modification to be identified was detyrosination¹, as most alpha-tubulins have a tyrosine at their terminus. This process affects microtubules more than dimers and leads to patches of detyronisation along the structure, regulating protein interactions and allowing subcellular compartments to be defined.^2,3  Polyglutamylation also occurs on several sites within the carboxy-terminal tails. However, to date, the most-studied alpha tubulin modification is related to acetylation of lysine 40 (K40).

 1. Gundersen, G. G., Khawaja, S. & Bulinski, J. C. Postpolymerization detyrosination of alpha-tubulin: a mechanism for subcellular differentiation of microtubules. J. Cell Biol. 105, 251-64 (1987).

2. Galjart, N. Plus-End-Tracking Proteins and Their Interactions at Microtubule Ends. Curr. Biol. 20, R528-R537 (2010).

3. Jiang, K. & Akhmanova, A. Microtubule tip-interacting proteins: a view from both ends. Curr. Opin. Cell Biol. 23, 94-101 (2011).

{{ptg:RelatedPrimaryAntibodies}}