Anticorps Recombinant de lapin anti-Alpha Tubulin

• Phare
• Validé par KD/KO

Alpha Tubulin Recombinant Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G7

N° de cat : 80762-1-PBS

Synonymes

TUBA1B, Tubulin alpha 1B chain, 1G7, Alpha-tubulin ubiquitous, EC:3.6.5.-

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Galerie de données de validation

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  WB analysis using 80762-1-RR (same clone as 80762-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80762-1-RR (Tubulin-Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80762-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 80762-1-RR (same clone as 80762-1-PBS)

  WB result of Alpha Tubulin antibody (80762-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alpha Tubulin transfected HEK-293 cells. This data was developed using the same antibody clone with 80762-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 80762-1-RR (same clone as 80762-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80762-1-RR (Alpha Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80762-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 80762-1-RR (same clone as 80762-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80762-1-RR (Alpha Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80762-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 80762-1-RR (same clone as 80762-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 80762-1-RR (Alpha Tubulin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80762-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 80762-1-RR (same clone as 80762-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using Alpha Tubulin antibody (80762-1-RR, Clone: 1G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80762-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 80762-1-RR (same clone as 80762-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse testis tissue using Alpha Tubulin antibody (80762-1-RR, Clone: 1G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 80762-1-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 80762-1-RR (same clone as 80762-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Alpha Tubulin antibody (80762-1-RR, Clone: 1G7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80762-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 80762-1-RR (same clone as 80762-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Tubulin antibody (80762-1-RR, Clone: 1G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80762-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00551-1

  Cytometric bead array standard curve of MP00551-1, Tubulin-Alpha Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80740-1-PBS. Detection antibody: 80762-1-PBS. Standard:Ag18034. Range: 0.098-50 ng/mL.

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  Affinity and Kinetic Characterization of 80762-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 80762-1-PBS against Human Alpha Tubulin was performed. The affinity constant is below 1 pM.

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  Affinity and Kinetic Characterization of 80762-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80762-1-RR against Human Alpha Tubulin were performed. The affinity constant is 0.708 nM.

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Informations sur le produit

80762-1-PBS cible Alpha Tubulin dans les applications de WB, IHC, IF/ICC, IF-P, IF-Fro, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Alpha Tubulin Protéine recombinante Ag18034
• Nom complet: tubulin, alpha 1b
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC009314
• Symbole du gène: Alpha Tubulin
• Identification du gène (NCBI): 10376
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha tubulin is useful for scientists across fields as an internal control due to its high, ubiquitous expression pattern.

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