Anticorps Polyclonal de lapin anti-Amphiphysin

• Phare
• Validé par KD/KO

Amphiphysin Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13379-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse brain using 13379-1-AP (same clone as 13379-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13379-1-AP (Amphiphysin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse lung using 13379-1-AP (same clone as 13379-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13379-1-AP (Amphiphysin antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 13379-1-AP (same clone as 13379-1-PBS)

  IP result of anti-Amphiphysin (IP:13379-1-AP, 4ug; Detection:13379-1-AP 1:1500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 13379-1-AP (same clone as 13379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13379-1-AP (Amphiphysin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 13379-1-AP (same clone as 13379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13379-1-AP (Amphiphysin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 13379-1-AP (same clone as 13379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13379-1-AP (Amphiphysin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 13379-1-AP (same clone as 13379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13379-1-AP (Amphiphysin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 13379-1-AP (same clone as 13379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13379-1-AP (Amphiphysin antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 13379-1-AP (same clone as 13379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13379-1-AP (Amphiphysin antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13379-1-PBS cible Amphiphysin dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Amphiphysin Protéine recombinante Ag4204
• Nom complet: amphiphysin
• Masse moléculaire calculée: 695 aa, 76 kDa
• Poids moléculaire observé: 115-125 kDa
• Numéro d’acquisition GenBank: BC034376
• Symbole du gène: AMPH
• Identification du gène (NCBI): 273
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Amphiphysin (AMPH), a synaptic vesicle-associated protein that is highly concentrated in nerve terminal, is an autoantigen of Stiff-Man syndrome with breast cancer. Amphiphysin contains a N-terminal BAR (Bin1/Amphiphysin/Rv167) domain followed by an endocytosis domain, and a C-terminal SH3 domain. Amphiphysin has been proposed to function as a linker between the clathrin coat and dynamin in the endocytosis of synaptic vesicles. It may also participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. (PMID: 8552632; 8245793; 10559861)

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