Anticorps Polyclonal de lapin anti-Amylase Alpha

Amylase Alpha Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12540-1-PBS

Synonymes

AMY2B, Amylase, alpha, 1,4-alpha-D-glucan glucanohydrolase 2B, Alpha amylase, Alpha amylase 2B

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Galerie de données de validation

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  WB analysis of mouse pancreas using 12540-1-AP (same clone as 12540-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12540-1-AP (Amylase, alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12540-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 12540-1-AP (same clone as 12540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12540-1-AP (Amylase, alpha antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12540-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 12540-1-AP (same clone as 12540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12540-1-AP (Amylase, alpha antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12540-1-PBS in a different storage buffer formulation.

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  IF Staining of rat pancreas using 12540-1-AP (same clone as 12540-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat pancreas tissue using Amylase Alpha antibody (12540-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12540-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12540-1-PBS cible Amylase Alpha dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Amylase Alpha Protéine recombinante Ag3260
• Nom complet: amylase, alpha 2B (pancreatic)
• Masse moléculaire calculée: 511 aa, 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC011179
• Symbole du gène: AMY2B
• Identification du gène (NCBI): 280
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AMY2B(alpha-amylase 2B) belongs to the glycosyl hydrolase 13 family. This protein is involved in the endohydrolysis of 1,4-alpha-glucosidic linkages in oligosaccharides and polysaccharides. The full length 58 kDa protein has a signal peptide with 15 amino acids. Human amylases (Amy1, Amy2A, and Amy2B) commonly have two potential N-glycosylation sites (N427 and N476) in their C-terminal region (S Takashima, J Amano, 2012). 12540-1-AP can recognize both AMY2A and AMY2B.

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