Anticorps Polyclonal de lapin anti-APP/Beta Amyloid

• Phare
• Validé par KD/KO

APP/Beta Amyloid Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

58

Avis (2)

N° de cat : 25524-1-AP

Synonymes

ABETA, ABPP, amyloid beta 1-42, Amyloid beta A4 protein, amyloid P, amyloid precursor protein, Amyloid-beta precursor protein, Amyloid-beta protein 40, Amyloid-beta protein 42, APP, APPI, Aβ1-42, beta Amyloid

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Galerie de données de validation

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  WB analysis using 25524-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25524-1-AP (APP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HAP1 using 25524-1-AP

  HAP1 (WT and APP KO) lysates prepared with RIPA buffer, 50μg protein loaded. 25524-1-AP incubated at 1:500 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.

• 

  WB analysis using 25524-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25524-1-AP (APP/Beta Amyloid antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 3T3-L1 using 25524-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 25524-1-AP (APP, C-Terminal antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25524-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25524-1-AP (beta Amyloid (C-Terminal) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HAP1 using 25524-1-AP

  HAP1 lysates prepared and IP of APP performed using 2.0 μg of 25524-1-AP coupled to protein A- Dynabeads beads. The Ponceau stained transfers of each blot are shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate.These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.

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  IHC staining of human gliomas using 25524-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25524-1-AP (APP, C-Terminal antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 25524-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25524-1-AP (APP, C-Terminal antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 25524-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25524-1-AP (APP, C-Terminal antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 25524-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25524-1-AP (APP, C-Terminal antibody at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules C6, cellules HeLa, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de gliome humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 41 publications below
• IHC: See 14 publications below
• IF: See 11 publications below

Informations sur le produit

25524-1-AP cible APP/Beta Amyloid dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: APP/Beta Amyloid Protéine recombinante Ag22408
• Nom complet: amyloid beta (A4) precursor protein
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC065529
• Symbole du gène: APP
• Identification du gène (NCBI): 351
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Aβ derives from APP via proteolytic cleavage by proteases called α-, β- and γ-secretase. The α-secretase cleavage precludes the formation of Aβ, while the β- and γ-cleavages generate APP components with amyloidogenic features. Amyloid beta A4 precursor protein(APP), encoded by APP gene which locate on human chromosome 21q, is a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. APP expressed in all fetal tissues and is pronounced in brain, kidney, heart and spleen, but weak in liver. Defects in APP are the cause of Alzheimer disease type 1 (AD1). Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms. This antibody can recognize several isoforms of both mature and immature amyloid beta (A4) precursor protein, including APP770, APP677, APP695, APP696, APP733, APP751, APP752, and APP639. APP can be cleaved into several chains, this antibody could recognize fragments C99, Amyloid-beta protein 42, Amyloid-beta protein 40, C83, P3(40), C80, Gamma-secretase C-terminal fragment 59, Gamma-secretase C-terminal fragment 57, Gamma-secretase C-terminal fragment 50, C31.

Publications

Species	Application	Title
mouse	IF	Neuron; Loss of PYCR2 Causes Neurodegeneration by Increasing Cerebral Glycine Levels via SHMT2.; Authors - Nathalie Escande-Beillard
human	WB	Small; Brain-Penetration and Neuron-Targeting DNA Nanoflowers Co-Delivering miR-124 and Rutin for Synergistic Therapy of Alzheimer's Disease.; Authors - Qin Ouyang
rat	IHC	Biomater Res; Controlled release of Clenbuterol from a hydroxyapatite carrier for the treatment of Alzheimer's Disease; Authors - Yi-Wen Lin
mouse	IHC	Redox Biol; Cognitive enhancement and neuroprotective effects of OABL, a sesquiterpene lactone in 5xFAD Alzheimer's disease mice model.; Authors - Jiang-Jiang Tang
mouse,rat	WB,IF	Free Radic Biol Med; The neuroprotective effects of phosphocreatine on amyloid beta 25-35-induced differentiated neuronal cell death through inhibition of AKT/GSK-3β /Tau/APP/CDK5 pathways in vivo and vitro; Authors - Jie Ai
human	IHC	Cancer Lett; LACTB suppresses melanoma progression by attenuating PP1A and YAP interaction.; Authors - Yawen Ma

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jorge (Verified Customer) (03-28-2023)

Test to evaluate the efficacy of the antibody. Tested on SH-SY5Y APPswe cells differentiated with 10 uM retic acid for 5 days. DAPT treatment 10 uM for 24h. Cells lysed with RIPA buffer. Loading different protein concentrations (ug). Tricine SDS-PAGE 16% acrylamide gel. Transfered on PVDF membrane. Blocking 5% milk. Dilution 1:4000 in TBS-Tw 0.05%.

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: Cell line SHSY5Y APPswe

APP/Beta Amyloid Antibody Western Blot validation (1:4000 dilution) in Cell line SHSY5Y APPswe (Cat no:25524-1-AP)

FH

JIMMY (Verified Customer) (04-15-2019)

This antibody detects APP in the brain of 12 months C57BL/6 mice.

• Applications: Western Blot,