Anticorps Recombinant de lapin anti-Angiogenin

Angiogenin Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242738D1

N° de cat : 85635-1-RR

Synonymes

ANG, ANG 1, ANG I, ANG1, ANGI

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Galerie de données de validation

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  WB analysis using 85635-1-RR

  Untreated and Brefeldin A treated HepG2 cells were subjected to SDS PAGE followed by western blot with 85635-1-RR (ANG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 85635-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ANG antibody (85635-1-RR, Clone: 242738D1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: Brefeldin A treated HepG2 cells,
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85635-1-RR cible Angiogenin dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Angiogenin Protéine recombinante Eg1930
• Nom complet: angiogenin, ribonuclease, RNase A family, 5
• Masse moléculaire calculée: 147 aa, 17 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC054880
• Symbole du gène: Angiogenin
• Identification du gène (NCBI): 283
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Angiogenin (ANG), an angiogenic ribonuclease, is a member of the vertebrate-specific, secreted RNASE superfamily. Angiogenin, originally identified as a tumor angiogenic factor, was related with the growth and metastasis of numerous tumors. Angiogenin has been proposed as a permissive factor for angiogenesis induced by other angiogenic factors, including vascular endothelial growth factor (VEGF), basic fibroblast growth factor, acidic fibroblast growth factor, and epidermal growth factor. Angiogenin production and secretion may be stimulated by hypoxia. Increased angiogenin serum levels have been associated with the incidence and severity of several human tumors, including HCC. It is a 17 kDa precursor which is cleaved to generate the 14 kDa mature protein.

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