Anticorps Polyclonal de lapin anti-Angiopoietin 1

• Phare
• Validé par KD/KO

Angiopoietin 1 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

Conjugaison

Non conjugué

N° de cat : 23302-1-PBS

Synonymes

ANGPT1, AGP1, AGPT, ANG 1, ANG1

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Galerie de données de validation

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  WB analysis using 23302-1-AP (same clone as 23302-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 23302-1-AP (Angiopoietin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23302-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 23302-1-AP (same clone as 23302-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 23302-1-AP (Angiopoietin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23302-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 23302-1-AP (same clone as 23302-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23302-1-AP (Angiopoietin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23302-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 23302-1-AP (same clone as 23302-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23302-1-AP (Angiopoietin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23302-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 23302-1-AP (same clone as 23302-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 23302-1-AP (Angiopoietin 1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23302-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 23302-1-AP (same clone as 23302-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 23302-1-AP (Angiopoietin 1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23302-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 23302-1-AP (same clone as 23302-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Angiopoietin 1 antibody (23302-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 23302-1-PBS in a different storage buffer formulation.

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Informations sur le produit

23302-1-PBS cible Angiopoietin 1 dans les applications de et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Angiopoietin 1 Protéine recombinante Ag18829
• Nom complet: angiopoietin 1
• Masse moléculaire calculée: 498 aa, 58 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC152411
• Symbole du gène: Angiopoietin 1
• Identification du gène (NCBI): 284
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin 1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin 1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer, and other tumors.

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