Anticorps Polyclonal de lapin anti-Angiopoietin 1

Angiopoietin 1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 27093-1-AP

Synonymes

AGP1, AGPT, ANG 1, ANG1, angiopoietin 1, ANGPT1, KIAA0003

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Galerie de données de validation

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  WB analysis using 27093-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27093-1-AP (Angiopoietin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27093-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27093-1-AP (Angiopoietin 1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of U-87 MG using 27093-1-AP

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 27093-1-AP (Angiopoietin 1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HUVEC, cellules HeLa, cellules MG U-87, tissu cardiaque de souris, tissu pulmonaire de rat, tissu pulmonaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

27093-1-AP cible Angiopoietin 1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Angiopoietin 1 Protéine recombinante Ag25786
• Nom complet: angiopoietin 1
• Masse moléculaire calculée: 498 aa, 58 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC152411
• Symbole du gène: ANGPT1
• Identification du gène (NCBI): 284
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin 1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin 1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer, and other tumors.

Publications

Species	Application	Title
human	WB	J Ethnopharmacol; Lycium barbarum polysaccharides attenuates high glucose-induced diabetic retinal angiogenesis by rescuing the expression of miR-15a-5p in RF/6A cells; Authors - Yafei Zhu