Anticorps Polyclonal de lapin anti-Angiopoietin 1

Angiopoietin 1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 27093-1-AP

Synonymes

ANGPT1, AGP1, AGPT, ANG 1, ANG1

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Galerie de données de validation

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  WB analysis using 27093-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27093-1-AP (Angiopoietin 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27093-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27093-1-AP (Angiopoietin 1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 27093-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27093-1-AP (Angiopoietin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 27093-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27093-1-AP (Angiopoietin 1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HUVEC using 27093-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using Angiopoietin 1 antibody (27093-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HUVEC, cellules HeLa, cellules MG U-87, tissu cardiaque de rat, tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HUVEC,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

27093-1-AP cible Angiopoietin 1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Angiopoietin 1 Protéine recombinante Ag25786
• Nom complet: angiopoietin 1
• Masse moléculaire calculée: 498 aa, 58 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC152411
• Symbole du gène: Angiopoietin 1
• Identification du gène (NCBI): 284
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin 1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin 1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer, and other tumors.

Publications

Species	Application	Title
human	WB	J Ethnopharmacol; Lycium barbarum polysaccharides attenuates high glucose-induced diabetic retinal angiogenesis by rescuing the expression of miR-15a-5p in RF/6A cells; Authors - Yafei Zhu
mouse	WB	Aging Cell; Phosphodiesterase inhibitor ameliorates senescent changes of renal interstitial pericytes in aging kidney; Authors - Hyung Duk Kim
rat	WB	Basic Res Cardiol; Investigating the cause of cardiovascular dysfunction in chronic kidney disease: capillary rarefaction and inflammation may contribute to detrimental cardiovascular outcomes; Authors - Siavash Beikoghli Kalkhoran
human	WB	Mater Today Bio; Doxorubicin-loaded PEGylated liposome modified with ANGPT2-specific peptide for integrative glioma-targeted imaging and therapy; Authors - Hongyan Li ; KD Validated
human	WB,IF	Adv Healthc Mater; Targeted Anti-Inflammatory Nanozymes with Pro-Angiogenic Activity for Myocardial Infarction Therapy; Authors - Wenling Yang

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