Anticorps Recombinant de lapin anti-Angiopoietin-2

Angiopoietin-2 Recombinant Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

240760D5

N° de cat : 83816-1-RR

Synonymes

Angpt2, angiopoietin 2, Ang2, Ang 2, Agpt2

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Galerie de données de validation

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  WB analysis using 83816-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83816-1-RR (Angiopoietin-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 83816-1-RR

  IP result of anti-Angiopoietin-2 (IP:83816-1-RR, 4ug; Detection:83816-1-RR 1:1500) with mouse lung tissue lysate 1200 ug.

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  IHC staining of mouse kidney using 83816-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83816-1-RR (Angiopoietin-2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 83816-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83816-1-RR (Angiopoietin-2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 83816-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83816-1-RR (Angiopoietin-2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 83816-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83816-1-RR (Angiopoietin-2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83816-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83816-1-RR against Mouse Angiopoietin-2 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: tissu splénique de souris, tissu pulmonaire de souris
• Résultats positifs en IP: tissu pulmonaire de souris,
• Résultats positifs en IHC: tissu rénal de souris, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83816-1-RR cible Angiopoietin-2 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: angiopoietin 2
• Masse moléculaire calculée: 56KD
• Poids moléculaire observé: 68kDa
• Numéro d’acquisition GenBank: NM_007426.4
• Symbole du gène: Angpt2
• Identification du gène (NCBI): 11601
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Angiopoietin-2 (ANGPT2, ANG-2) is as a regulator of vessel enlargement, and ANGPT2 inhibition prevented pathological vessel enlargement (PMID: 34758631). ANGPT2 destabilizes vessels and promotes angiogenesis through antagonism of ANGPT1 (PMID: 34758631). Ang2 was initially identified by homology with Ang1 and is expressed predominantly by endothelial cells, where it is stored in intracellular secretory granules called Weibel-Palade bodies (WPB) and promptly released after endothelium activation. Like Ang1, Ang2 binds to Tie2 receptor, but with different pathophysiological effects. While Ang1 fosters endothelial stabilization, Ang2 can antagonize Ang1, blocking Tie2 activation and leading to vessel destabilization (PMID: 31756341).

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