Anticorps Polyclonal de lapin anti-Angiotensinogen/AGT

• Phare
• Validé par KD/KO

Angiotensinogen/AGT Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11992-1-PBS

Synonymes

AGT, Angiotensinogen, Serpin A8, SERPINA8, Ang IV

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Galerie de données de validation

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  WB analysis using 11992-1-AP (same clone as 11992-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11992-1-AP (Angiotensinogen antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11992-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 11992-1-AP (same clone as 11992-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11992-1-AP (Angiotensinogen antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11992-1-PBS in a different storage buffer formulation.

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  IP experiment of human plasma using 11992-1-AP (same clone as 11992-1-PBS)

  IP result of anti-Angiotensinogen (IP:11992-1-AP, 4ug; Detection:11992-1-AP 1:1000) with human plasma lysate 1600 ug. This data was developed using the same antibody clone with 11992-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 11992-1-AP (same clone as 11992-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11992-1-AP (Angiotensinogen antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11992-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 11992-1-AP (same clone as 11992-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11992-1-AP (Angiotensinogen antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11992-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11992-1-AP (same clone as 11992-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Angiotensinogen antibody (11992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11992-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 11992-1-AP (same clone as 11992-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Angiotensinogen Polyclonal antibody (11992-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11992-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11992-1-PBS cible Angiotensinogen/AGT dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Angiotensinogen/AGT Protéine recombinante Ag2632
• Nom complet: angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
• Masse moléculaire calculée: 485 aa, 53 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC011519
• Symbole du gène: AGT
• Identification du gène (NCBI): 183
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Angiotensinogen is a precursor of angiotensin II (Ang II), is expressed and synthesized largely in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. It has a key role in mediating vascular constriction and regulating salt and fluid homeostasis. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene also have been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.

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