Anticorps Polyclonal de lapin anti-Angptl4

Angptl4 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 51109-1-AP

Synonymes

Hepatic fibrinogen/angiopoietin-related protein, ANGPTL4 N-terminal chain, ANGPTL4 C-terminal chain, Angiopoietin-related protein 4, Angiopoietin-like protein 4

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Galerie de données de validation

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  WB analysis using 51109-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51109-1-AP (Angptl4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 51109-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 51109-1-AP (Angptl4 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 51109-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 51109-1-AP (Angptl4 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 51109-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 51109-1-AP (Angptl4 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 51109-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 51109-1-AP (Angptl4 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 51109-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 51109-1-AP (Angptl4 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 51109-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 51109-1-AP (Angptl4 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris,
• Résultats positifs en IHC: tissu rénal humain, tissu rénal de rat, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

51109-1-AP cible Angptl4 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Angptl4 Protéine recombinante Ag0613
• Nom complet: angiopoietin-like 4
• Masse moléculaire calculée: 405 aa, 45 kDa
• Numéro d’acquisition GenBank: BC078944
• Symbole du gène: Angptl4
• Identification du gène (NCBI): 362850
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Angiopoietin-like protein 4 (ANGPTL4), a member of the angiopoietin-like protein subfamily, is a secretory protein that functions as an important modulator of glucose and lipid metabolism. ANGPTL4 inhibits lipoprotein lipase (LPL)-dependent lipolysis thereby limiting the uptake of free fatty acids. Overexpression of ANGPTL4 results in hypertriglyceridemia, while ANGPTL4 deficiency suppresses foam formation in macrophages and protects against atherosclerosis. ANGPTL4 may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabetes. ANGPTL4 has three functional domains: the signal peptide, the coiled-coil (N-terminal chain, CCD), and the fibrinogen-like (C-terminal chain, FLD) domains. ANGPTL4 is a glycosylated secreted protein (fANGPTL4, 45-65 kDa). fANGPTL is then rapidly post-translationally cleaved into an N-terminal coiled-coil domain (nANGPTL4, ~ 15 kDa) and a C-terminal fibrinogen-like domain (cANGPTL4, ~ 35 kDa)(PMID: 37300585 ).

Publications

Species	Application	Title
human,mouse	IF	Theranostics; Advancing the clinical assessment of glomerular podocyte pathology in kidney biopsies via super-resolution microscopy and angiopoietin-like 4 staining; Authors - Xiaojing Liu ; KO Validated

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