Anticorps Monoclonal anti-Annexin A10

Annexin A10 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

1A7A11

N° de cat : 66869-1-Ig

Synonymes

Annexin 10, Annexin 14, annexin A10, ANX14, ANXA10

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Galerie de données de validation

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  WB analysis of pig stomach using 66869-1-Ig

  pig stomach tissue were subjected to SDS PAGE followed by western blot with 66869-1-Ig (Annexin A10 antibody) at dilution of 1:2100 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-29 using 66869-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66869-1-Ig (Annexin A10 antibody) at dilution of 1:2100 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 66869-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 66869-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66869-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66869-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 66869-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 66869-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 66869-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 66869-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 66869-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 66869-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon cancer using 66869-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Annexin A10 antibody (66869-1-Ig, Clone: 1A7A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human colon cancer using 66869-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Annexin A10 antibody (66869-1-Ig, Clone: 1A7A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu d'estomac de porc, cellules HT-29
• Résultats positifs en IHC: tissu d'estomac humain, tissu de cancer du côlon humain, tissu de cancer du pancréas humain, tissu de côlon humain, tissu pancréatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du côlon humain,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

66869-1-Ig cible Annexin A10 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Annexin A10 Protéine recombinante Ag27098
• Nom complet: annexin A10
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC007320
• Symbole du gène: ANXA10
• Identification du gène (NCBI): 11199
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ANXA10, member of the Annexin family, is a calcium-and-phospholipid-binding protein. It is implicated in multiple physiological processes, including growth regulation, cell division, apoptosis, and differentiation. ANXA10 was recently observed to be upregulated in Barrett's esophagus, oral cancers, pancreatic cancer and colon cancer.

Publications

Species	Application	Title
human	WB	Discov Oncol; Identification of KRT16 and ANXA10 as cell cycle regulation genes for lung adenocarcinoma based on self-transcriptome sequencing of surgical samples and TCGA public data mining; Authors - Wen-Jian Liu

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