Anticorps Monoclonal anti-Annexin A3

Annexin A3 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

1A11E4

N° de cat : 66405-1-Ig

Synonymes

ANXA3, 1A11E4, 35 alpha calcimedin, 35-alpha calcimedin, Annexin 3

---

Galerie de données de validation

• 

  WB analysis using 66405-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 66405-1-Ig

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66405-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 66405-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66405-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 66405-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66405-1-Ig (Annexin A3 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 66405-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66405-1-Ig (Annexin A3 antibody at dilution of 1:200 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: cellules HCT 116, cellules Caco-2, cellules DU 145, cellules HEK-293, cellules HeLa, cellules HepG2, cellules L02, cellules SW-1990, tissu cardiaque humain, tissu splénique humain
• Résultats positifs en IHC: tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

66405-1-Ig cible Annexin A3 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Annexin A3 Protéine recombinante Ag2381
• Nom complet: annexin A3
• Masse moléculaire calculée: 322 aa, 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC000871
• Symbole du gène: Annexin A3
• Identification du gène (NCBI): 306
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Annexin A3 (Anxa3), also named as lipocortin3, placental anticoagulant protein3 (PAP-III), belongs to annexin family. Annexins are calcium-dependent phospholipids-binding high-abundant intracellular proteins. ANXA3 has been reported to play crucial roles in a diverse range of molecular and cellular processes, including ion channel regulation, anticoagulation, anti-inflammation, vesicle traffick-ing, signal transduction, cell migration, and cell proliferation. It also exhibits important roles in cancer progression, metastasis, and drug resistance.

Publications

Species	Application	Title
mouse	WB,IHC,IF	Oncogene; Epstein-Barr virus causes vascular abnormalities in epithelial malignancies through upregulating ANXA3-HIF-1α-VEGF pathway; Authors - Yuanyuan Chen ; KO Validated

{{ptg:RelatedPrimaryAntibodies}}