Anticorps Monoclonal anti-Annexin A4

Annexin A4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2c

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A10B5

N° de cat : 60288-2-PBS

Synonymes

35-beta calcimedin, Annexin A4, Annexin IV, Annexin-4, ANXA4, Chromobindin-4, Endonexin I, Lipocortin IV

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Galerie de données de validation

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  WB analysis of COLO 320 using 60288-2-Ig (same clone as 60288-2-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60288-2-Ig (Annexin IV antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 60288-2-Ig (same clone as 60288-2-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 60288-2-Ig (Annexin IV Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 60288-2-Ig (same clone as 60288-2-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60288-2-Ig (Annexin IV antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 60288-2-Ig (same clone as 60288-2-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 60288-2-Ig (Annexin IV antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 60288-2-Ig (same clone as 60288-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60288-2-Ig (Annexin IV antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 60288-2-Ig (same clone as 60288-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60288-2-Ig (Annexin IV antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60288-2-Ig (same clone as 60288-2-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 60288-2-Ig(Annexin IV antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

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Informations sur le produit

60288-2-PBS cible Annexin A4 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2c
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Annexin A4 Protéine recombinante Ag0130
• Nom complet: annexin A4
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC000182
• Symbole du gène: Annexin A4
• Identification du gène (NCBI): 307
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Annexin A4 (Anxa4) is one of the Ca2 +-regulated and phospholipid-binding annexin superfamily proteins, and is located at 2p13. Anxa4 has a potential role in diagnosis, prognosis, and treatment of certain cancers. Annexin A4 shares 45 to 59% identity with other members and possibly interacts with ATP. Annexin A4 has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. The Annexin A4 protein is expressed in a wide range of tissues, associated with polarized epithelial cells and is proposed to be involved in the regulation of chloride ions across the epithelial membrane.

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