Anticorps Recombinant de lapin anti-DNA

DNA Recombinant Antibody for IHC, FC (Intra), Dot Blot, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

n/a

Applications

IHC, FC (Intra), Dot Blot, ELISA

Conjugaison

Non conjugué

CloneNo.

250864A1

N° de cat : 85854-1-RR

Synonymes

circular DNA, linear DNA, single-stranded DNA

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Galerie de données de validation

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  IHC staining of human ovary tumor using 85854-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 85854-1-RR (Anti-DNA antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 85854-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 85854-1-RR (Anti-DNA antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 85854-1-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85854-1-RR (Anti-DNA antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 85854-1-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85854-1-RR (Anti-DNA antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using 85854-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.2 ug N/a Anti-DNA Recombinant Antibody (85854-1-RR, Clone:250864A1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Dot Blot experiment using 85854-1-RR

  Dot blot analysis was used to confirm the specificity of 85854-1-RR anti-DNA antibody. samples were spotted onto NC and probed with antibody at 5 µg/ml.The amount of sample (μg) spotted is indicated next to each row.

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Applications testées

• Résultats positifs en IHC: tissu placentaire humain, tissu d'amygdalite humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules HeLa,
• Résultats positifs en Dot Blot: N/A,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:800-1:3200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.2 ug per 10^6 cells in a 100 µl suspension
• DOT BLOT: DOT BLOT : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85854-1-RR cible DNA dans les applications de IHC, FC (Intra), Dot Blot, ELISA et montre une réactivité avec des échantillons n/a

• Réactivité: n/a
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: unknow
• Nom complet: DNA
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNA antibodies are a type of antibody that can specifically bind to DNA and are mainly used to detect and study DNA-related biological processes. This antibody can recognize single-stranded DNA, linear DNA, and circular DNA.

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