Anticorps Monoclonal anti-Apolipoprotein AI

Apolipoprotein AI Monoclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, souris

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

1C9G5

N° de cat : CL594-66206

Synonymes

APOA1, apolipoprotein A I, Apo-AI, ApoAI, ApoA I

---

Galerie de données de validation

• 

  IF Staining of HepG2 using CL594-66206

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66206 (Apolipoprotein AI antibody) at dilution of 1:100.

• 

  IF Staining of L02 using CL594-66206

  Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using CoraLite®594-conjugated Apolipoprotein AI antibody (CL594-66206, Clone: 1C9G5 ) at dilution of 1:100, CL488-Phalloidin (green).

• 

  FC experiment of HepG2 using CL594-66206

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Apolipoprotein AI (CL594-66206, Clone:1C9G5) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en IF/ICC: cellules HepG2, cellules L02
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66206 cible Apolipoprotein AI dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Apolipoprotein AI Protéine recombinante Ag21920
• Nom complet: apolipoprotein A-I
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 26-28 kDa
• Numéro d’acquisition GenBank: BC005380
• Symbole du gène: APOA1
• Identification du gène (NCBI): 335
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.

{{ptg:RelatedPrimaryAntibodies}}