Anticorps Recombinant de lapin anti-Apoe

Apoe Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240786C3

N° de cat : 83728-4-PBS

Synonymes

apolipoprotein E, AI255918, 240786C3

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Galerie de données de validation

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  WB analysis using 83728-4-RR (same clone as 83728-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83728-4-RR (Apoe antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83728-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83728-4-RR (same clone as 83728-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83728-4-RR (Apolipoprotein E antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83728-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83728-4-RR (same clone as 83728-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83728-4-RR (Apolipoprotein E antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83728-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 83728-4-RR (same clone as 83728-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83728-4-RR (Apolipoprotein E antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83728-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 83728-4-RR (same clone as 83728-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83728-4-RR (Apolipoprotein E antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83728-4-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 83728-4-RR (same clone as 83728-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 83728-4-RR (Apolipoprotein E antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83728-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83728-4-RR (same clone as 83728-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Apoe antibody (83728-4-RR, Clone: 240786C3 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83728-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83728-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83728-4-RR against Mouse Apolipoprotein E were performed. The affinity constant is 0.793 nM.

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Informations sur le produit

83728-4-PBS cible Apoe dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: Apolipoprotein E
• Masse moléculaire calculée: 36kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: NM_001305819.1
• Symbole du gène: Apoe
• Identification du gène (NCBI): 11816
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Apolipoprotein E (Apoe) is a core component of plasma lipoproteins that participates in diverse biological processes, including plasma lipoprotein metabolism, intracellular cholesterol utilization, cell growth, immunoregulation, and neuronal growth and repair (PMID: 17854398). Mouse apoE, like apoE4, contains the equivalent of Arg-112 and Glu-255, but lacks the critical Arg-61 equivalent (it contains Thr-61). Thus, mouse apoE does not display apoE4 domain interaction (PMID: 11553788).

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